About this book
General Description of the Volume: This volume, as do the other "Enzyme Kinetics and Mechanism" volumes in the "Methods in Enzymology" series, provides treatment of dynamic and chemical approaches for investigating enzyme catalysis and regulation, as well as designing metabolic inhibitors. It will greatly interest those involved in enzyme chemistry, metabolic control, and drug design. It should also interest those developing commercial applications for enzymes whose properties have been re-engineered using recombinant DNA technology and site-directed mutagenesis. General Description of the Series: The critically acclaimed laboratory standard for more than forty years, "Methods in Enzymology" is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. Now with more than 300 volumes (all of them still in print), the series contains much material still relevant today - truly an essential publication for researchers in all fields of life sciences. It covers mechanisms of enzyme catalysis and inhibition. It also describes enzyme structure and function. It talks about the regulatory control of enzymes. It covers action of catalytic antibodies and ribozymes.
Contents
General Approaches to Biological Catalysis: C.A. Fierke and G.G. Hammes, Transient Kinetic Approaches to Enzyme Mechanisms. K.A. Johnson, Rapid Quench Kinetic Analysis of Polymerases, Adenosinetriphosphatases, and Enzyme Intermediates. R.G. Duggleby, Analysis of Enzyme Progress Curves by Nonlinear Regression. B.V. Plapp, Site-Directed Mutagenesis: A Tool for Studying Enzyme Catalysis. Inhibitors as Mechanistic Probes: H.J. Fromm, Reversible Enzyme Inhibitors as Mechanistic Probes. S.E. Szedlacsek and R.G. Duggleby, Kinetics of Slow and Tight-Binding Inhibitors. W.W. Cleland, Kinetic Method for Determination of Dissociation Constants of Metal Ion(Nucleotide Complexes. B.F.Cooper and F.B. Rudolph, Product Inhibition Applications. K.L. Rebholz and D.B. Northrop, Kinetics of Iso Mechanisms. R.B. Silverman, Mechanism-Based Enzyme Inactivators. A. Radzicka and R. Wolfenden, Transition State and Multisubstrate Analog Inhibitors. Isotopic Probles of Enzyme Action: I.A. Rose, Partition Analysis: Detecting Enzyme Reaction Cycle Intermediates. W.W. Cleland, Isotope Effects: Determination of Enzyme Transition State Structure. B.J. Bahnson and J.P. Klinman, Hydrogen Tunneling in Enzyme Catalysis. L.S. Mullins and F.M. Raushel, Positional Isotope Exchange as Probe of Enzyme Action. M.-D. Tsai, R.-T. Jiang, T. Dahnke, and Z. Shi, Manipulating Phosphorus Stereospecificity of Adenylate Kinase by Site-Directed Mutagenesis. F.C. Wedler, Equilibrium Isotope Exchange in Enzyme Catalysis. D.N. Silverman, Proton Transfer in Carbonic Anhydrase Measured by Equilibrium Isotope Exchange. Kinetics of Specialized Systems: J.D. Stewart, I. Lee, B.A. Posner, and S.J. Benkovic, Expression of Properly Folded Catalytic Antibodies in Escherichia coli. K.E. Neet, Cooperativity in Enzyme Function: Equilibrium and Kinetic Aspects. M.K. Jain, M.H. Gelb, J. Rogers, and O.G. Berg, Kinetic Basis for Interfacial Catalysis by Phospholipase A2. Author Index. Subject Index.
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Biography
Daniel Lee Purich has been at the forefront of biochemistry research for more than 25 years. He was awarded the National Institutes of Health Research Career Development Award from 19771982, the Plous Teaching Award (the University of California Santa Barbara Campus-Wide Teaching Award) in 1977, has been a member of the National Institutes of Health Biochemistry Study Section from 19821985, and a member of the Journal of Biological Chemistry Editorial Board from 19811986. He has been a member of the American Society of Biological Chemists, the American Chemical Society, the New York Academy of Sciences, the Biochemical Society, and the American Society for Cell Biology. Dr. Purich is currently a Professor and Chairman of the Department of Biochemistry and Molecular Biology at the Florida College of Medicine. He is the author and editor of numerous scientific publications.
