The objectives of this Second Edition of "Biotechnology: A Laboratory Course" remain unchanged: to create a text that consists of a series of laboratory exercises that integrate molecular biology with protein biochemistry techniques while providing a continuum of experiments. The course begins with basic techniques and culminates in the utilization of previously acquired technical experience and experimental material. Two organisms, Sacchaomyces cerevisiae and Escherichia coli, a single plasmid, and a single enzyme are the experimental material, yet the procedures and principles demonstrated are widely applicable to other systems. This text will serve as an excellent aid in the establishment or instruction of introductory courses in the biological sciences. All exercises and appendixes have been updated. It includes new exercises on: polymerase chain reaction; Beta-Galactosidase detection in yeast colonies; and western blotting. New procedures are introduced for: large-scale plasmid isolation; yeast transformation; DNA quantitation. New appendixes are added, one of which provides details on accessing biological information sites on the Internet (World Wide Web). It covers use of non-radioactive materials and provides easy access to microbial cultures. Laboratory exercises are student tested for seven years.
This is an excellent laboratory guide for a laboratory course in basic techniques in molecular biology. It is also an excellent course text for instructors who want experiments to work and obtain joy from seeing their students being excited from success and gaining confidence in the laboratory. --Kalidas Shetty in JOURNAL OF FOOD BIOCHEMISTRY
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