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Gene Probes: A Practical Approach, Volume 1: Preparation

Handbook / Manual

Series: Gene Probes: A Practical Approach Volume: 1

Edited By: BD Hames and SJ Higgins

284 pages, 7 b/w photos, 24 illus

IRL Press

Paperback | Oct 1995 | #45820 | ISBN: 0199634009
Availability: Usually dispatched within 2-3 weeks Details
NHBS Price: £69.99 $85/€78 approx
Hardback | Dec 1995 | #45819 | ISBN: 0199634017
Out of Print Details

About this book

The preparation of gene probes is a core application in molecular biology creating essential tools for genetic research. This volume together with its forthcoming companion volume, Gene Probes 2, provides a timely and important resource for all researchers in this field. Gene Probes 1 focuses on the wide range of techniques now available for the purification, synthesis, labelling, detection, and quantitation of probes, including antibody probes. Gene Probes 2 will concentrate on describing techniques for specific research goals. Contents List: Strategies for using gene probes; Preparation of nucleic acids for use as probes; DNA radiolabelling and detection; Non-radioactive labelling of DNA probes; Preparation of oligodeoxynucleotide probes; Probe preparation by PCR; Synthesis of labelled riboprobes; Antibody probes


Introduction; 1. Strategies for using gene probes; 2. Preparation of nucleic acids for use as probes; 3. DNA radiolabelling and detection; 4. Non-radioactive labelling of DNA probes; 5. Preparation of synthetic oligodeoxynucleotide probes; 6. Probe preparation of PCR; 7. Synthesis of labelled riboprobes; 8. Antibody probes; Appendix 1; Appendix 2

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