Capillary Electrophoresis Guidebook

About this book

Capillary Electrophoresis Guidebook offers both newcomers and experienced research workers hands-on guidance to performing capillary electrophoresis. It provides sufficient practical advice to permit you to develop and optimize your own separations, along with extensive troubleshooting sections to overcome practical difficulties. The book contains operating instructions for standard commercially available instruments and includes guidelines for activities such as changing capillaries, method development, quantitative procedures, optimizing sensitivity, and the validation of methods. Review chapters written by leading experts discuss micellar electrokinetic capillary chromatography, capillary gel electrophoresis, advanced sampling techniques, and electrochromatography. Important application areas such as the analysis of proteins, peptides, amino acids, pharmaceuticals, chiral compounds, and nucleic acids are also treated.

Contents

Part I: General Guidelines to the Operation of Capillary Electrophoresis Methods and Instrumentation. Fundamentals of Capillary Electrophoresis Theory. Standard Commercial Instrument Description. Typical Operating Procedures. Method Development/Optimization. Quantitation Procedures. Optimization of Precision in Quantitative Analysis. Optimization of Sensitivity. Method Validation. Fraction Collection. Troubleshooting. Quick Guide to Running a Successful Separation. Part II: Applications of Capillary Electrophoresis and Specific Technologies. Micellar Electrokinetic Capillary Chromatography. Capillary Gel Electrophoresis. Chiral Separations by Capillary Electrophoresis. Capillary Electrochromatography. Application and Limits of Sample Stacking in Capillary Electrophoresis. Analysis of Bases Nucleotides and (Oligo)nucleotides by Capillary Electrophoresis. Application of Capillary Electrophoresis to Pharmaceutical Analysis. Separation of Peptides and Protein Digests by Capillary Electrophoresis. Additional Applications of Capillary Electrophoresis. Part III: Appendices. Index.

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