Gene Mapping Protocols

About this book

An unprecedented collection of all the most up-to-date techniques for gene isolation and mapping, including the latest methods for gene characterization using database analyses. This collection of thoroughly tested recipes also includes chapters for the computational analysis of novel cDNA sequences with up-to-the-minute information on basic sequence analysis, sequence similarity searches, exon detection and similarity searches, and the prediction of gene function. Its state-of-the-art methods constitute indispensable tools for all scientists engaged in the search for specific disease genes, or in the general advancement of the human genome project.

Contents

Gene Mapping Goes from FISH to Surfing the Net John Valdes and Danilo A. Tagle. Linkage Analysis of Genetic Disorders Eugene W. Taylor Jianfeng Xu Ethylin Wang Jabs and Deborah A. Meyers. Gene Ordering and Localization by Linkage Analysis R. E. March. Gene Mapping Using Somatic Cell Hybrids David P. Kelsell and Nigel K. Spurr. Gene Mapping by FISH Rafael Espinosa III and Michelle M. Le Beau. Probe Ordering and Distancing by FISH Marian Kroef Hans Dauwerse and Jim Landegent. Physical Mapping by Pulsed- Field Gel Electrophoresis John Maule. Construction and Use of YAC Contigs in Disease Regions Stephen Horrigan and Carol Westbrook. Construction and Use of Cosmid Contigs Nicholas Fairweather. Use of Dinucleotide Polymorphism Analyses in Physical Mapping Jeff Fairman and Lalitha Nagarajan. Mapping Expressed Sequence Tags (ESTs) by Multiplexing PCR Reactions from Hybrid Cell Panels and Detecting Fluorescently Labeled Products A. Scott Durkin Donna R. Maglott and William C. Nierman. Gene Isolation by Exon Trapping David B. Krizman. Isolation of Coding Sequences from Genomic Regions Using Direct Selection Richard G. Del Mastro and Michael Lovett. Isolation of cDNAs Using the YAC Hybridization Screen Method Carrie Fidler and Jacqueline Boultwood. Detection and Isolation of Differentially Expressed Genes by Differential Display Weimin Zhu and Peng Liang. Chemical Crosslinking Subtraction (CCLS) Ian N. Hampson and Lynne Hampson. Gene Mapping and Isolation: Access to Databases Martin J. Bishop. Software for Genetic Linkage Analysis: An Update Stephen P. Bryant. Exon Detection by Similarity Searches Jean-Michel Claverie. Index.

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