Books  Animal & General Biology  Genetics 

In Vitro Mutagenesis Protocols

Handbook / Manual

Series: Methods in Molecular Biology Volume: 57

By: MK Trower

400 pages, Illus

Humana Press

Spiralbound | Dec 1996 | #52791 | ISBN: 0896033325
Availability: Usually dispatched within 1-2 weeks Details
NHBS Price: £48.99 $60/€55 approx

About this book

In this volume experts from industrial and academic laboratories describe easily reproducible and up-to-date procedures for site directed and random mutagenesis. Site-directed protocols include those based on strand selection, PCR (including "splicing by overlap extension" and the "megaprimer" procedure), the ligase chain reaction, positive antibiotic selection, unique restriction site elimination, gapped heteroduplex formation, and solid-phase capture with the biotin/strepavidin system. Many can be used with virtually any double-stranded DNA plasmid. The bookcovers genesis, linker scanning mutagenesis, nested deletion mutagenesis, and a specialized E coli mutator strain.

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