Flow Cytometry Protocols

About this book

Leading researchers and experimentalists update the field of flow cytometry with detailed accounts of their many new, improved, and well-tested protocols and applications. Among the cutting-edge methods presented here are techniques for the detection of terminal transferase ion leukemia, intracellular cytokines, intracellular/intranuclear antigens in leukemia and tumors, and cyclins. Protocols for performing functional studies include methods for assessing viability, transmembrane potential, oxidative metabolism, environmental particulate uptake, drug resistance, and cellular pharmacokinetics. The methods for examining DNA address cell cycle analysis, DNA/RNA analysis of solid malignancies, solid-tumor DNA ploidy analysis, apoptosis, and fluorescent in situ hybridization. Protocols for chromosome sorting and 5-6 color analysis are included as advanced methods. The techniques in this volume take advantage of the new instrumentation and probes that have recently been developed to advance the field of flow cytometry and improve standard methods.

Contents

Basics of Flow Cytometry, G.Radcliff and M. J. Jaroszeski. Detection of Terminal Transferase in Leukemia, E. Paietta. The Use of Flow Cytometry to Detect Intracellular Cytokine Production in Individual Cells, B. E. Crucian and R. H. Widen. Detection of Intracellular/Intranuclear Antigens, R. H. Widen. Detection of Intracellular/Intranuclear Antigens in Tumor Cells, R. H. Widen and J. L. Becker. Detection of Cyclins in Individual Cells by Flow and Laser Scanning Cytometry, G. Juan and Z. Darzynkiewicz. Estimation of Cell Viability by Flow Cytometry, J. L. Weaver. Flow Cytometric Detection of Fluorescent Redistributional Dyes for Measurement of Cell Transmembrane Potential, M. K. Tanner and S. R. Wellhausen. Flow Cytometric Analysis of Macrophage Oxidative Metabolism Using DCFH, A. Imrich and L. Kobzik. Measurement of Environmental Particulate Uptake by Lung Cells Using Flow Cytometry, B. Stringer and L. Kobzik. Estimation of Drug Resistance by Flow Cytometry, A. Aszalos and J. L. Weaver. Flow Cytometric Monitoring of Cellular Pharmacokinetics, M. S. Dordal. Quantification of Xenoprotein Electroinsertion in Mammalian Cells, K. El Ouagari and J. Teissie. Flow Cytometry Quantification of Electropermeabilization, M.-P. Rols and J. Teissie. Flow Cytometric Detection and Quantitation of Cell-Cell Electrofusion Products, M. J. Jaroszeski, R. Gilbert, and R. Heller. Cell Cycle Analysis of Asynchronous Populations, M. G. Ormerod. Simultaneous DNA/RNA Analysis of Solid and Hematoreticular Malignancies, K. Steck and A. K. El-Naggar. Solid Tumor DNA Ploidy Analysis, K. Steck and A. K. El-Naggar. FISHing for Cytokines: Methodology Combining Flow Cytometry and In Situ Hybridization, K. J. Pennline. Analysis of Apoptosis by Flow Cytometry, W. Gorczyca, M. R. Melamed, and Z. Darzynkiewicz. Chromosome Sorting and Analysis by FACS, S. P. Monard. Simultaneous Five-Six Color Multiparameter Analysis, K. J. Pennline. Index.

Customer Reviews