989 pages, 600 illustrations
Guide to the enormous range of techniques available to the researcher for both the genetic and physical mapping of the genome, as well as the sequencing and analysis of DNA. Each chapter presents a state-of-the-art review of a methodology. Methods are fully described and evaluated; their advantages and disadvantages discussed; and their suitability for different investigations considered. Step by step protocols, including computer analyses, are given for 123 essential experimental procedures.
Introduction Family Studies Complex Studies Constructing And Using Genetic Maps Automated Map Construction Polymorphic Markers DNA Typing Part II: Physical Mapping I: Cytogenetic Analysis: Introduction Conventional Cytogenetics Solid Tumor Cytogenetics In Situ Hybridisation Chromosome Painting Chromosome Microdissection Chromosome Sorting And Analysis Digital Microscopy Part III: Physical Mapping II: Long-Range Mapping And Gene Isolation: Introduction Somatic Cell Hybrid Approaches To Genome Mapping Long-Range Physical Mapping Integrating Genetic And Physical Maps Strategies For Rapid Isolation Of Genes From Genomic DNA:Cdna Techniques Screening Cdna Libraries By Transient Expression In Mammalian Cells Part IV: Sequencing And Sequence Analysis: Introduction Sequencing Strategies Template Amplification And Purification Sequencing Chemistries Electrophoresis Sequence Labelling And Detection Automation Mutation Analysis Repeat Analysis Part V: Other Model Systems: Introduction Mouse Mapping Somatic Gene Therapy Drosophila Melanogaster Caenorhabditis Elegan Saccharomyces Cerevisiae Escherichia Coli Problems In Plant Genome Analysis Arabidopsis Thaliana Part VI: Internet Resources: Introduction Internet Resources And How To Use Them Unix Survival Kit Databases And Genome Resource Centers Appendices Materials, Media And Solutions Preparation Of Blood Bottles And Processing Of Blood Samples Commercial Suppliers Other Resources Useful Addresses And Internet Connections Basic Data On Human And Other Genomes Catalogue Of Mapped Human Disease Genes Mouse Gene Knock-Out Tables Chromosome Aberrations Associated With Cancer Glossary Index
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