Books  Animal & General Biology  Biochemistry & Molecular Biology 

Protein and Peptide Analysis by Mass Spectrometry

Handbook / Manual

Series: Methods in Molecular Biology Volume: 61

Edited By: JR Chapman

352 pages, Illus

Humana Press

Hardback | Dec 1996 | #55973 | ISBN: 0896033457
Availability: Usually dispatched within 1-2 weeks Details
NHBS Price: £115.50 $145/€136 approx

About this book

Leading practitioners authoritatively describe the newest and most effective spectrometric techniques for the analysis of proteins and peptides. The areas covered range from the elucidation of primary and secondary protein structure and the rapid identification of proteins using database techniques to methods for sequencing, as well as methods for the quantitative determination of peptides. Other chapters provide detailed information on the analysis of glycoproteins and glycopeptides and on the use of mass spectrometry to probe the interactions of proteins, both covalent and noncovalent.

The 21 Chapters cover the whole range of techniques, with enough detail about the physical and technical background to satisfy and enable the critical user without swamping him or her; all illustrated with a wide range of cutting-edge applications...The book closes with a valuable series of appendices (acronyms, mass and abundance values, fragmentation patterns etc)...there is no lightweight material or padding in this important and timely book - The European Peptide Society Newsletter useful to newcomers. - SGM Quarterly


Contents

Mass Spectrometry in the Analysis of Peptides and Proteins Past and Present Peter Roepstorff. Mass Spectrometry: Ionization Methods and Instrumentation John R. Chapman. Charged Derivatives for Peptide Sequencing Using a Magnetic Sector Instrument Joseph Zaia. New Instrumental Approaches to Collision-Induced Dissociation Using a Time-of-Flight Instrument Bernhard Spengler. Mass Spectrometric Quantification of Neuropeptides Dominic M. Desiderio. The Identification of Electrophoretically Separated Proteins by Peptide Mass Fingerprinting John S. Cottrell and Chris W. Sutton. Liquid Chromatography/Mass Spectrometry for the Analysis of Protein Digests Thomas Covey. Structural Analysis of Protein Variants Yoshinao Wada. Noncovalent Interactions Observed Using Electrospray Ionization Brenda L. Schwartz David C. Gale and Richard D. Smith. Protein Secondary Structure Investigated by Electrospray Ionization Carol V. Robinson. The Effect of Detergents on Proteins Analyzed by Electrospray Ionization Rachele R. Ogorzalek Loo Natalie Dales and Philip C. Andrews. Posttranslational Modifications Analyzed by Automated Protein Ladder Sequencing Rong Wang and Brian T. Chait. Rapid Analysis of Single-Cysteine Variants of Recombinant Proteins Thomas W. Keough Yiping Sun Bobby L. Barnett Martin P. Lacey Mark D. Bauer Ellen S. Wang and Christopher R. Erwin. Disulfide Bond Location in Proteins Yiping Sun Mark D. Bauer Thomas W. Keough and Martin P. Lacey. Determination of Loading Values and Distributions for Drugs Conjugated to Proteins and Antibodies by MALDI-MS and ESI-MS Marshall M. Siegel. Hydrophobic Proteins and Peptides Analyzed by Matrix-Assisted Laser Desorption/Ionization Kevin L. Schey. Analysis of Glycoproteins and Glycopeptides Using Fast-Atom Bombardment Geert Jan Rademaker and Jane Thomas-Oates. Identification of Cleaved Oligosaccharides by Matrix-Assisted Laser Desorption/Ionization David J. Harvey. Structural Characterization of Protein Glycosylation Using HPLC/ Electrospray Ionization Mass Spectrometry and Glycosidase Digestion Christine A. Settineri and Alma L. Burlingame. Analysis of Complex Protein and Glycoprotein Mixtures by Electrospray Ionization Mass Spectrometry with Maximum Entropy Processing Brian N. Green Therese Hutton and Serge N. Vinogradov. The Use of Databases in Searching the Literature of Biological Mass Spectrometry Ronald W. A. Oliver and Michael P. Carrier. Appendices. Index.

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