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Epitope Mapping Protocols

Handbook / Manual

Series: Methods in Molecular Biology Volume: 66

Edited By: GE Morris

504 pages, B/w photos, line illus

Humana Press

Spiralbound | Dec 1996 | #55975 | ISBN: 0896033759
Availability: Usually dispatched within 1-2 weeks Details
NHBS Price: £108.00 $137/€127 approx

About this book

Provides an extensive range of expertly written protocols for the creation of epitope libraries and their analysis.


Contents

Overview: Choosing a Method for Epitope Mapping Glenn E. Morris. Crystallographic Studies of Antigen-Antibody Interactions Frederick A. Saul and Pedro M. Alzari. Epitope Mapping Antibody-Antigen Complexes by Nuclear Magnetic Resonance Spectroscopy Irina Kustanovich and Anat Zvi. Mapping Epitopes on Antigens by Immunodiffusion in Gel Giuseppe A. Molinaro and William C. Eby. A Simple Solid-Phase Competition Assay with Labeled Antigen Masahide Kuroki. Epitope Mapping by Antibody Competition: Methodology and Evaluation of the Validity of the Technique Socrates J. Tzartos. Epitope Mapping by Surface Plasmon Resonance in the BIAcore Berit Johne. Identifying Residues in Antigenic Determinants by Chemical Modification N. Martin Young and Raymond P. Oomen. Epitope Mapping by Differential Chemical Modification of Antigens Hans Rudolf Bosshard. Epitope Mapping by Proteolysis of Antigen-Antibody Complexes: Protein Footprinting Ronald Jemmerson. Proteolytic Fragmentation for Epitope Mapping Maria R. Mazzoni Nickolai O. Artemyev and Heidi E. Hamm. Epitope Mapping by Chemical Fragmentation Glenn E. Morris. Probing Antibody-Antigen Interactions by Mass Spectrometry Yingming Zhao and Brian T. Chait. Epitope Mapping Using Multipin Peptide Synthesis Stuart J. Rodda N. Joe Maeji and Gordon Tribbick. SPOT Synthesis: Epitope Analysis with Arrays of Synthetic Peptides Prepared on Cellulose Membranes Ronald Frank and Heike Overwin. Tea Bag Synthesis of Positional Scanning Synthetic Combinatorial Libraries and Their Use for Mapping Antigenic Determinants Clemencia Pinilla Jon R. Appel and Richard A. Houghten. Epitope Mapping Using Phage-Displayed Peptide Libraries Diane Dottavio. Reiterative Screening of Phage-Display Peptide Libraries with Antibodies Alexander Pereboev and Glenn E. Morris. Homolog Scanning Lin-Fa Wang. Epitope Mapping Using an Oligonucleotide Replacement Method Hannah Alexander. Epitope Mapping by Region-Specified PCR Mutagenesis Takehiko Shibata and Masayuki Ikeda. Random Fragment Libraries Using Yeast Expression Plasmid Serge B'nichou and GeneviSve Inchausp'. Epitope Mapping Using Random Fragment Expression Libraries in l Phages Hartmut Porzig and Kenneth D. Philipson. Random Fragment Libraries Displayed on Filamentous Phage Lin-Fa Wang and Meng Yu. Epitope Mapping by Expression of Restriction Enzyme or PCR Fragments in Bacterial Plasmids Johannes A. Lenstra and Arnoud H. M. Van Vliet. Epitope Mapping of Protein Antigens by Expression-PCR (E-PCR) David E. Lanar Kevin C. Kain and Henry B. Burch. Epitope Mapping on Extracellular Domains of Cell-Surface Proteins Using Exonuclease III Thomas Br?mmendorf Antonius Plagge and Ullrich Treubert. An Improved Method for Mapping Epitopes of Recombinant Antigens by Transposon Mutagenesis Steven G. Sedgwick Brian A. Morgan Nguyen thi Man and Glenn E. Morris. Incomplete Polypeptides of In Vitro Translation for Epitope Localization Lisa Djavadi-Ohaniance and Bertrand Friguet. T-Cell Epitope Mapping with Synthetic Peptides and Peripheral Blood Mononuclear Cells Stuart J. Rodda. Use of Natural or Selected Mutants and Variants for Epitope Mapping Glenn E. Morris. Production of Panels of Monoclonal Antibodies by the Hybridoma Method Nguyen thi Man and Glenn E. Morris. Production of Phage-Display Antibodies for Epitope Mapping Jenny Walker and George Banting. Index.

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