Books  Animal & General Biology  Biochemistry & Molecular Biology 

Protein Sequencing Protocols

Handbook / Manual

Series: Methods in Molecular Biology Volume: 64

Edited By: BJ Smith

360 pages, Illus

Humana Press

Hardback | Dec 2002 | Edition: 2 | #135634 | ISBN: 0896039757
Availability: Usually dispatched within 1-2 weeks Details
NHBS Price: £123.00 $160/€138 approx

About this book

Bryan John Smith updates his much-acclaimed first edition with new and updated techniques for determining the sequence of proteins and peptides. This edition includes not only novel approaches to the validation of quality assurance methods, reflecting the current importance of biopharmaceuticals, but also offers a guide to analysis of protein sequence information via the powerful new tools of bioinformatics. Comprehensive and up-to-date, "Protein Sequencing Protocols, Second Edition", provides for both novice and expert investigators alike a ready source of easy-to-follow protocols that simplify choosing the most appropriate method for protein sequence determination.

Review of the first edition: "The overall result is a concise and highly informative laboratory manual aimed at both operators and users of protein analysis facilities providing an understanding of what techniques involve, how samples should be prepared and in what form and amounts they should be submitted to achieve the most successful data. This publication has certainly proved to be a valuable asset to my laboratory and I would recommend it not only to those in protein sequencing and mass spectrometry laboratories, but also anyone with protein chemistry applications and interests."-Molecular Biotechnology||From Reviews of the First Edition... "...full of valuable information..." -Biochemical Education "...provides very useful resources for those embarking on protein identification or characterization projects..." -FEBS Letters " extremely useful compendium of protein analysis protocols..." -Nahrung-Food "...well organized and accessible..." -Journal of the American Chemical Society


Strategies for Handling Polypeptides on a Micro-Scale Bryan John Smith and Paul Tempst SDS Polyacrylamide Gel Electrophoresis for N-Terminal Protein Sequencing Bryan John Smith Two-Dimensional Polyacrylamide Gel Electrophoresis for the Separation of Proteins for Chemical Characterization Michael J. Dunn Electroblotting of Proteins from Polyacrylamide Gels for Chemical Characterization Michael J. Dunn Enzymatic Cleavage of Proteins Bryan John Smith Chemical Cleavage of Polypeptides Bryan John Smith Enzymatic Preparation and Isolation of Glycopeptides David J. Harvey Analytical and Micropreparative Capillary Electrophoresis of Peptides Alan J. Smith High-Performance Liquid Chromatography On-Line Derivative Spectroscopy for the Characterization of Peptides with Aromatic Amino Acid Residues Christoph W. Turck Hydrolysis of Samples for Amino Acid Analysis Ian Davidson Amino Acid Analysis: Precolumn Derivatization Methods G. Brent Irvine and Ian Davidson Post Column Amino Acid Analysis Alan J. Smith Amino Acid Analysis Using Pre-Column Derivatization with 6-Aminoquinolyl-N-Hydroxysuccinimidyl Carbamate: Analysis of Hydrolyzed Proteins and Electroblotted Samples Steven A. Cohen Amino Acid Analysis in Protein Hydrolysates Using Anion Exchange Chromatography and IPAD Detection Petr Jandik, Jun Cheng, and Nebojsa Avdalovic d-Amino Acid Analysis Andrea Scaloni, Maurizio Simmaco, and Francesco Bossa Validation of Amino Acid Analysis Methods Andrew J. Reason Electrospray Mass Spectrometry of Peptides, Proteins, and Glycoproteins Fiona M. Greer and Howard R. Morris Peptide Mass Fingerprinting Using MALDI-TOF Mass Spectrometry Darryl J. C. Pappin Nanoelectrospray Tandem Mass Spectrometry and Sequence Similarity Searching for Identification of Proteins from Organisms with Unknown Genomes Anna Shevchenko, Shamil Sunyaev, Adam Liska, Peer Bork, and Andrej Shevchenko Direct Identification of Proteins in Ultracomplex Mixtures: Applications to Proteome Analysis David M. Schieltz and John R. Yates, III Identification of PTH-Amino Acids by HPLC Gregory A. Grant and Mark W. Crankshaw Protein Sequencer Maintenance and Troubleshooting Bryan Dunbar N-Terminal Protein Sequencing for Special Applications Philip J. Jackson Identification of Phosphorylation Sites by Edman Degradation Laurey Steinke and Richard G. Cook Validation of Protein Sequencing in a Regulated Laboratory Philip J. Jackson and Neil Dodsworth Automated C-Terminal Protein Sequence Analysis Using the Alkylated-Thiohydantoin Method David R. Dupont, Sylvia W. Yuen, and Kenneth S. Graham Chemical Modifications of Proteins as an Aid to Sequence Analysis Alex F. Carne Deblocking of N-Terminally Modified Proteins Hisashi Hirano and Roza M. Kamp Deblocking of Proteins Containing N-Terminal Pyroglutamic Acid Jacek Mozdzanowski Identification of Sites of Protein Glycosylation David J. Harvey Analysis of Sites of Protein Phosphorylation Alastair Aitken and Michele Learmonth Quantitation and Location of Disulfide Bonds in Proteins Alastair Aitken and Michele Learmonth Getting the Most from Your Protein Sequence Richard R. Copley and Robert B. Russell Appendix 1: Letter Codes, Structures, Masses, and Derivatives of Amino Acids James P. Turner Appendix 2: Protein Consensus Sequence Motifs Alastair Aitken Index

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