A comprehensive collection of essential, time-tested recipes for successful protein fractionation and purification in any experimental circumstance. The protocols give step-by-step instructions on how to select a source for the protein of interest, how to obtain a usable initial extract, how to purify the protein from that extract using both chemical and molecular methods, and how to dry and store the purified protein. Protein Purification Protocols provides all that is needed to design and carry out a successful purification program. It helps both experienced and novice investigators to clarify and define their purification problems and then provides a comprehensive set of tools for a practical solution.
...includes some very useful contributions. - Angewandte Chemie This book is recommended for protein chemists, whether beginner or professional. There is something in this book for everyone. It is an excellent working book as well as an excellent reference. - INFORM-American Oil Chemists Society Doonan's handy compendium...holds its own against Methods in Enzymology [and] Current protocols in Protein Science... will be valuable to keep around the laboratory for most graduate students in biochemistry, and indeed for more senior practitioners of biotechnology as well. - Biopharm Overall, a well presented utilitarian bench manual and a worthwhile purchase for novice and established practitioner alike - Society for General Microbiology Quarterly
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