Glycoanalysis Protocols

About this book

Glycoanalysis Protocols, 2nd ed., makes available to all protein scientists, and particularly those working in today's pharmaceuticals industry, the most advanced and reproducible glycoanalysis techniques. These detailed, up-to-date, and proven analytical methods cover the areas of glycoprotein macromolecular structural analysis, oligosaccharide profiling, lipid conjugate characterization, microorganism structure determination, and proteoglycan function. They also include advanced analytical techniques in biotechnology during the production of recombinant glycoproteins and other therapeutics. These protocols will well serve anyone starting work on the analysis of glycoproteins, as well as experienced investigators seeking to carry their expertise to higher levels of accomplishment.

Contents

Characterization of Protein Glycosylation E. F. Hounsell. Analytical Methods for the Study of O-GlcNAc Glycoproteins and Glycopeptides K. D. Greis and G. W. Hart. Analysis of Asparagine- Linked Oligosaccharides by Sequential Lectin-Affinity Chromatography K. Yamamoto T. Tsuji and T. Osawa. Exoglycosidase Sequencing of N-Linked Glycans by the Reagent Analysis Method (RAAM) S. Prime and T. Merry. HPAE-PAD Analysis of Monosaccharides Released by Exoglycosidase Digestion Using the Carbopac MA1 Column M. Weitzhandler J. Rohrer J. R. Thayer and N. Avdalovic. HPLC and HPAEC of Oligosaccharides and Glycopeptides M. J. Davies and E. F. Hounsell. Analysis of N- and O-Glycans by Pyridylamination S. Natsuka and S. Hase. Polyacrylamide Gel Electrophoresis of Fluorophore-Labeled Carbohydrates from Glycoproteins J. C. Klock and C. M. Starr. Analysis of Glycosaminoglycans and Proteoglycans C. C. Rider. The Use of Cell and Organ Culture for the Study of Secreted Mucins A. P. Corfield A. Aslam S. Wood B. Singh and C. Paraskeva. Purification of Gastrointestinal Mucins and Analysis of Their O-Linked Oligosaccharides B. J. Campbell and J. M. Rhodes. TLC in Structure and Recognition Studies of Glycosphingolipids J. M?thing. Strategies for Preliminary Characterization of Novel Amphoteric Glycosphingolipids R. D. Dennis G. Lochnit and R. Geyer. Analysis of the Carbohydrate and Lipid Components of Glycosylphosphatidylinositol Structures A. Treumann M. Lucia S. G?ther P. Schneider and M. A. J. Ferguson. Conformational Analysis of Biantennary Glycopeptides with a Resonance Energy Transfer Technique K. B. Lee P. Wu L. Brand and Y. C. Lee. Index.

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