Biochemical and Biotechnological Applications of Electrospray Ionization Mass Spectrometry

About this book

Presents new ways of creating ions of polar and labile biopolymeric species with remarkable ease and efficiency. Introduces better characterization of proteins and other biological molecules. Presents major fundamental issues in such areas as immunology, biochemistry, and natural toxins. Demonstrates methods used to resolve the data from various biological process investigations into useful information. Provides an application-driven approach, presenting valuable insights into the experiments in the volume.

Contents

Electrospray: A Popular Ionization Technique for Mass Spectrometry; Analytical Characteristics of the Electrospray Ionization Process; Electrospray Ion Formation: Desorption Versus Desertion; Liquid Chromatography with Electrospray Ionization Tandem Mass Spectrometry: Profiling Carbohydrates in Whole Bacterial Cell Hydrolysates; Identification of a Cytoplasmic Petidoglycan Precursor in Antibiotic-Resistant Bacteria; Structural Characterization of Prokaryotic Glycans and Oligosaccharides; Structural Characterization of Lipopolysaccharides from Pseudomonas aeruginosa Using Capillary Electrophoresis Electrospray Ionization Mass Spectrometry and Tandem Mass Spectrometry; Determining Structures and Functions of Surface Glycolipids in Pathogenic Haemophilus Bacteria by Electrospray Ionization Mass Spectrometry; Electrospray Ionization Mass Spectrometry for Structural Characterization of the Lipid A Component in Bacterial Endotoxins; Microcolumn Liquid Chromatography-Electrospray Ionization Tandem Mass Spectrometry: Analysis of Immunological Samples; Identification of Proteins from Two-Dimensional Electrophoresis Gels by Peptide Mass Fingerprinting; Lipid Metabolism of the Mosquito Pathogen Lagenidium giganteum and Its Hosts: Glycerophospholipids and Hydroxy Fatty Acids; Electrospray Ionization Mass Spectrometry of Biological Macromolecules: Characterization of Polyunsaturated Phospholipid Remodeling in Mammalian Cells by High-Performance Liquid Chromatography-Electrospray Ionization Mass Spectometry; Application of Electrospray Ionization Mass Spectrometry to the Analysis of Oligodeoxynucleotides; Noncovalent Complexes of Nucleic Acids and Proteins Studied by Electrospray Ionization Mass Spectrometry; Analysis of Xanomeline, a Potential Drug for Alzheimer's Disease, by Electrospray Ionization Tandem Mass Spectrometry; High-Performance Liquid Chromatography with Atmospheric Pressure Ionization Tandem Mass Spectrometry as a Tool in Quantitative Bioanalytical Chemistry; Analysis of Diarrhetic Shellfish Poisoning Toxins and Metabolites in Plankton and Shellfish by Ion-Spray Liquid Chromatography-Mass Spectrometry; Identification of Active-Site Residues in Glycosidases; Electrospray Ionization Mass Spectrometric Investigation of Signal Transduction Pathways: Determination of Sites of Inducible Protein Phosphorylation in Activated T Cells; Characterization of Recombinant Glycoproteins from Chinese Hamster Ovary Cells; Complications in the Determination of Molecular Weights of Proteins and Peptides Using Electrospray Ionization Mass Spectrometry; Use of Hyphenated Liquid-Phase Analyses-Mass Spectrometric Approaches for the Characterization of Glycoproteins Derived from Recombinant DNA; From Protein Primary Sequence to the Gamut of Covalent Modifications Using Mass Spectrometry; Analysis of Biomolecules Using Electrospray Ionization-Ion-Trap Mass Spectrometry and Laser Photodissociation; Atmospheric Pressure Ionization Liquid Chromatography-Mass Spectrometry for Environmental Analysis

Customer Reviews