Books  Animal & General Biology  Biochemistry & Molecular Biology 

DNA Sequencing Strategies: Automated and Advanced Approaches

Handbook / ManualOut of Print

Edited By: W Ansorge, H Voss and J Zimmerman

202 pages, Photos, data charts, tables

John Wiley & Sons

Spiralbound | Nov 1996 | #60298 | ISBN: 0471136832
Out of Print Details

About this book

This outstanding lab bench reference to the technology of DNA sequencing offers a collection of concise sequencing strategies and cloning protocols. Concentrates on the most up-to-the-minute automated methods and advanced approaches. Preparing DNA for sequencing, sequencing single- doubled-stranded DNA and their variations, how to optimise the primers used, preparation of DNA sequencing gels and the actual collection of results, labelling of DNA fragments for sequencing and data analysis are among the topics covered.


Contents

Sequencing strategies and cloning protocols; preparation of templates; DNA sequencing protocols; selection, synthesis and purification of primers; preparation of DNA sequencing gels and electrophoresis; computer analysis; selected literature.

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