Books  Animal & General Biology  Biochemistry & Molecular Biology 

Recombinant Protein Production in Plants

Handbook / Manual

Series: Methods in Molecular Biology Volume: 84

By: C Cunningham and A Porter

356 pages, Illus

Humana Press

Hardback | Dec 1997 | #66302 | ISBN: 0896033902
Availability: Usually dispatched within 1-2 weeks Details
NHBS Price: £115.50 $150/€129 approx

About this book

This authoritative collection of basic and advanced protocols permits biotechnologists to use plants rather than single cell fermentation systems for expressing recombinant proteins. The protocols provide clear step-by-step instructions for the cloning, expression, and analysis of a wide variety of recombinant proteins in plants, and for the cloning of genes into a number of different plant species. The book also describes promising experimental techniques that will become increasingly important in the future, and includes techniques for the characterization of medically important proteins expressed in plants.


Rice Transformation by Agrobacterium Infection X. Cheng R. K. Sardana and I. Altosaar. Barley as a Producer of Heterologous Protein L. Mannonen K. Aspegren A. Ritala H. Simola and T. H. Teeri. Introducing and Expressing Genes in Legumes A. D. Bavage M. P. Robbins L. Sk>t and K. J. Webb. Use of the GUS Reporter Gene J. Wilkinson and K. Lindsey. Expression of Recombinant Proteinase Inhibitors in Plants D. Michaud and T. C. Vrain. Detection of Recombinant Viral Coat Protein in Transgenic Plants H. Steinkellner and I. Korschineck. Synthesis of Recombinant Human Cytokine GM-CSF in the Seeds of Transgenic Tobacco Plants R. K. Sardana P. R. Ganz A. Dudani E. S. Tackaberry X. Cheng and I. Altosaar. Tobamovirus Vectors for Expression of Recombinant Genes in Plants T. H. Turpen and S. J. Reinl. Single-Chain Fv Antibodies Expressed in Plants U. Conrad U. Fiedler O. Artsaenko and J. Phillips. Characterization and Applications of Plant-Derived Recombinant Antibodies R. Fischer J. Drossard Y. Liao and S. Schillberg. Production of Recombinant Antibodiesin Plant Suspension Cultures J. Drossard Y.-C. Liao and R. Fischer. Production of Foreign Proteins in Tobacco Cell Suspension Culture V. Gomord A.-C. Fitchette-Lain' L.-A. Denmat D. Michaud and L. Faye. Transient Gene Expression in Plant Protoplasts S. Schillberg S. Zimmermann D. Pr?fer D. Schuman and R. Fischer. Stability of Recombinant Proteins in Plants D. Michaud T. C. Vrain V. Gomord and L. Faye. Trafficking and Stability of Heterologous Proteinsin Transgenic Plants J. A. Napier G. Richard and P. R. Shewry. Screening for Transgenic Lines with Stableand Suitable Accumulation Levels of a Heterologous Protein M. De Neve H. Van Houdt A.-M. Bruyns M. Van Montagu and A. Depicker. Manipulation of Photosynthetic Metabolism M. A. J. Parry S. P. Colliver P. J. Madgwick and M. J. Paul. Quantification of Heterologous Protein Levelsin Transgenic Plants by ELISA A.-M. Bruyns M. De Neve G. De Jaeger C. De Wilde P. Rouz' and A. Depicker. Analysis of N- and O-Glycosylation of Plant Proteins A.-C. Fitchette-Lain' L.-A. Denmat P. Lerouge and L. Faye. Two-Dimensional Electrophoresis: A Tool for Protein Separation and Processing for Microsequencing N. P. E. Reddy and M. Jacobs. Index.

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