Cell Culture Labfax

About this book

An easy-to-use rapid reference compendium of essential data, specifically designed to meet the research needs of laboratory scientists.

Contents

Cell lines - characteristics of widely used cell lines; initiation and maintenance of cultures - typical calculations, culture of specialized cells, growth equations; cell counting - typical calculations from Coulter counter and haemocytometer; production and maintenance of hybridomas - mice strains, fusion partners, fusiogens, immunization, selection media; reference data; techniques for characterizing culture parameters - hymidine labelling, autobiography, immunohistochemistry, staining, chromosome spreads/analysis, DNA fingerprinting; cell separation - reference to widely used techniques; cell cloning - commonly used vectors; media formulations - for growth,maintenance and cell selection; serum/serum-free formulations - recipes and growth promoting characteristics; cryopreservation - solutions used, liquid nitrogen storage; buffers used for cell maintenance; microcarriers - characteristics of those available for cell growth; growth factors - availability for use in cell cultures - characteristics for growth promotion; analysis of culture media - reference to methods for the determination of the major components in media; products from cells in culture - characteristics of those routinely produced and their commercial value; equipment for cell culture - growth vessels, fermenters, incubators; safety aspects of handling cells; terms used in cell culture - glossary of terminology; commercial suppliers; professional bodies.

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