Phytoplankton Pigments in Oceanography: Guidelines to Modern Methods

About this book

Will be useful not only in the fields of marine and freshwater ecology, but also for specialized tasks such as ocean-colour satellite signals, monitoring aquatic environments and algal blooms, and standardizing methods used by environmental protection agencies.

Contents

Part 1 Literature reviews - background to modern oceanography: development of pigment methods for oceanography; introduction to marine phytoplankton and their pigment signatures; metabolism and function of photosynthetic pigments; application of pigment methods to oceanography; pigment processes in the sea - a selected bibliography. Part 2 Experimental results of SCOR working group: simple procedures for growing SCOR reference microalgal cultures; preparation of chlorophyll standards; preparation of carotenoid standards; caluation methods and solvents for pigment extraction; filtration and storage of pigments of microalgae; simple isocratic HPLC methods for chlorophylls and degradation products; high-resolution HPLC system for chlorophylls and carotenoids of marine phytoplankton; qualitative and quantitative HPLC analysis of SCOR reference algal cultures; comparson between spectrophotometric, fluorometric and HPLC methods for chlorophyll analysis. Part 3 Guidelines for HPLC systems, sampling and selecting methods for pigments: guidelines for selecting and setting up an HPLC system and laboratory; calibration mathods for HPLC; guidelines for collection and pigment analysis of field samples.

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