Mitotic Metaphase Chromosome Preparation from Peripheral Blood for High Resolution. Chromosome Preparation from Hematological Malignancies. Meiotic Chromosome Preparation. Preparation of Chromosomes for Scanning Electron Microscopy. Immortalized Cell Lines: Chromosome Preparation and Banding. Chromosome Banding and Identification: Absorption Staining. Chromosome Banding and Identification: Fluorescence. Chromosome Banding: Stain Combinations for Specific Regions. Inhibition of Chromosome Condensation. Analysis of Chromosomes with Restriction Endonucleases and DNase Hypersensitivity. Automated Karyotype Analysis. Bivariate Chromosome Analysis Using a Commercial Flow Cytometer. Chromosome Sorting by Flow Cytometry: Production of DNA Libraries and Gene Mapping. Electrophoretic Karyotype Analysis: Pulse Field Gel Electrophoresis. Immunofluorescence Techniques Applied to Mitotic Chromosome Preparations. Immunocytochemical Techniques Applied to Meiotic Chromosome Preparations. Immortalized Cell Lines: Their Creation and Use in Gene Mapping. Oligonucleotide-Primed In Situ DNA Synthesis: An Alternative to In Situ Hybridization for Gene Mapping and the Investigation of Genome Organization. Electron Microscopic Localization of In Situ Hybrids. Protocols for Chromosome Mediated Gene Transfer: Selection Strategies, Transgenome Analysis, Enrichment Cloning, and Mapping. Construction of Chromosome Specific Libraries of Yeast Artificial Chromosome Recombinants from Somatic Hybrid Cell Lines. Yeast Artificial Chromosome Recombinants in a Global Strategy for Chromosome Mapping: Amplification of Internal and Terminal Fragments by PCR, and Generation of Fingerprints. Chromosome Dissection and Cloning. Molecular Analysis of Chromosome Aberrations in Hematological Malignancies: Southern Hybridization. Molecular Analysis of Chromosome Aberrations: In Situ Hybridization. Alu- and L1-Primed PCR-Generated Probes for Non-Isotopic In Situ Hybridization. Chromosome Substructure Investigation: Telomeres. Index.