Books  Animal & General Biology  Biochemistry & Molecular Biology 

Chromosome Analysis Protocols

Handbook / Manual

Series: Methods in Molecular Biology Volume: 29

Edited By: JR Gosden

528 pages, Illus

Humana Press

Spiralbound | Dec 1994 | #33058 | ISBN: 0896032434
Availability: Usually dispatched within 1-2 weeks Details
NHBS Price: £123.00 $155/€145 approx

About this book

Although emphasizing the use of human material, most of the techniques are readily applicable to other animal chromosomes.

...a very worthwhile book...invaluable for those trying to venture into the area of molecular cytogenetics...Provides a ready source of both old and new cytogenetic methods and contains a wealth of troubleshooting tips from experienced workers in the field. - Modern Pathology


Contents

Mitotic Metaphase Chromosome Preparation from Peripheral Blood for High Resolution. Chromosome Preparation from Hematological Malignancies. Meiotic Chromosome Preparation. Preparation of Chromosomes for Scanning Electron Microscopy. Immortalized Cell Lines: Chromosome Preparation and Banding. Chromosome Banding and Identification: Absorption Staining. Chromosome Banding and Identification: Fluorescence. Chromosome Banding: Stain Combinations for Specific Regions. Inhibition of Chromosome Condensation. Analysis of Chromosomes with Restriction Endonucleases and DNase Hypersensitivity. Automated Karyotype Analysis. Bivariate Chromosome Analysis Using a Commercial Flow Cytometer. Chromosome Sorting by Flow Cytometry: Production of DNA Libraries and Gene Mapping. Electrophoretic Karyotype Analysis: Pulse Field Gel Electrophoresis. Immunofluorescence Techniques Applied to Mitotic Chromosome Preparations. Immunocytochemical Techniques Applied to Meiotic Chromosome Preparations. Immortalized Cell Lines: Their Creation and Use in Gene Mapping. Oligonucleotide-Primed In Situ DNA Synthesis: An Alternative to In Situ Hybridization for Gene Mapping and the Investigation of Genome Organization. Electron Microscopic Localization of In Situ Hybrids. Protocols for Chromosome Mediated Gene Transfer: Selection Strategies, Transgenome Analysis, Enrichment Cloning, and Mapping. Construction of Chromosome Specific Libraries of Yeast Artificial Chromosome Recombinants from Somatic Hybrid Cell Lines. Yeast Artificial Chromosome Recombinants in a Global Strategy for Chromosome Mapping: Amplification of Internal and Terminal Fragments by PCR, and Generation of Fingerprints. Chromosome Dissection and Cloning. Molecular Analysis of Chromosome Aberrations in Hematological Malignancies: Southern Hybridization. Molecular Analysis of Chromosome Aberrations: In Situ Hybridization. Alu- and L1-Primed PCR-Generated Probes for Non-Isotopic In Situ Hybridization. Chromosome Substructure Investigation: Telomeres. Index.

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