RNA Isolation and Characterization Protocols

About this book

Innovative biological investigators offer a powerful and highly useful collection of up-to-date methods for the isolation of RNA from a variety of sources, including bacterial, plant, and mammalian cells. These fine-tuned protocols take both the experienced and inexperienced investigator through the nuances of RNA manipulation, from extraction to in vitro translation. The book also includes protocols for the analysis and characterization of isolated RNA species and extensive troubleshooting hints and tips to ensure easy reproducibility. RNA Isolation and Characterization Protocols is the single best collection of easily reproducible, step-by-step RNA techniques now available for today's bench scientists.

Contents

Introduction to Isolating RNA D. E. Macfarlane and C. E. Dahle. Large and Small Scale RNA Preparations from Eukaryotic Cells W. Uckert W. Walther and U. Stein. An Improved Rapid Method of Isolating RNA from Cultured Cells D. B. Batt G. G. Carmichael and Z. Liu. Isolating RNA with the Cationic Surfactant Catrimox-14 C. E. Dahle and D. E. Macfarlane. RNA Extraction from Formalin-Fixed and Paraffin-Embedded Tissues G. Stanta S. Bonin and R. Perin. Extraction and Purification of RNA from Plant Tissues Enriched in Polysaccharides S.H. Cheng and J. R. Seemann. Isolation of Plant Mitochondrial RNA from Green Leaves F. Ye W. O. Abel and R. Reski. Extraction of RNA from Fresh and Frozen Blood B. D. M. Theophilus. Isolation of Total RNA from Bacteria J. Heptinstall. Isolation of Total RNA from Tissues or Cell Lines: Visualization in Gel T. Mukhopadhyay and J. A. Roth. Isolation of Messenger RNA S. Bryant and D. L. Manning. UV Spectrometric Analysis of Ribonucleic Acids R. Rapley and J. Heptinstall. Formaldehyde Gel Electrophoresis of Total RNA R. D. Dennis G. Lochnit and R. Geyer. Preparation of RNA Dot-Blots R. Hodge. Nonradioactive Northern Blotting R. Low. The Use of RNA Probes for the Analysis of Gene Expression: Northern Blot Hybridization and Ribonuclease Protection Assay D. Belin. Analysis of RNA by Northern Blotting Using Riboprobes R. A. K. Srivastava. RNA Quantitative Analysis from Fixed and Paraffin-Embedded Tissues G. Stanta S. Bonin and R. Utrera. Quantitative Analysis of RNA Species by PCR and Solid-Phase Minisequencing A. Suomalainen and A.-C. Syvanen. Preparation of Tissue Sections and Slides for mRNA Hybridization G. Terenghi. Detecting mRNA in Tissue Sections with Digoxigenin-Labeled Probes G. Terenghi. One-Tube RT-PCR with Sequence Specific Primes U. Pfeffer. Identification of Differentially Exposed Genes by Nonradioactive Differential Display Messenger RNA T. C. G. Bosch and J. U. Lohmann. Characterization of RNA Using Continuous RT-PCR Coupled with ELOSA F. Mallet G. Oriol and B. Mandrand. Gene Expression Analysis by CD-RT-PCR E. de Kant. Primer Extension Analysis of mRNA M. Walmsley M. Leonard and R. Patient. S1 Mapping Using Single-Stranded DNA Probes S. Viville and R. Mantovani. Measurements of Rate of Transcription in Isolated Nuclie by Nuclear Run-Off Assay R. A. K. Srivastava and G. Schonfeld. Transcription In Vitro Using Bacteriophage RNA Polymerases E. T. Schenhborn. In Vitro Translation of Messenger RNA in a Rabbit Reticulocyte Lysate Cell-Free System L. Olliver and C. D. Boyd. In Vitro Translation of Messenger RNA in a Wheat Germ Extract Cell-Free System L. Olliver A. G.-Rabie and C. D. Boyd. The Xenopus Egg Extract Translation System G. M. Matthews and A. Colman. Purification and Chracterization of Viral dsRNA Genome Profiles by Crosshybridization L.-A. Martin and P. P. C. Mertens. Index.

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