Books  Animal & General Biology  Biochemistry & Molecular Biology 

Cell Separation: A Practical Approach

Handbook / Manual

Series: Practical Approach Series Volume: 193

Edited By: Derek Fisher, Gillian E Francis and David Rickwood

288 pages, Illus

IRL Press

Paperback | Nov 1998 | #83406 | ISBN: 019963579X
Availability: Usually dispatched within 2-3 weeks Details
NHBS Price: £69.99 $88/€83 approx
Hardback | Dec 1998 | #83404 | ISBN: 0199635803
Out of Print Details

About this book

Isolating specific cells or fractionating viable cell populations is an essential step of many of the techniques used across a wide range of biological and related disciplines. This text aims to inform those working in all areas of the biological sciences of the methods which are available to them and which is likely to be the most useful. The contributing authors have provided a comprehensive guide to the methods used to prepare cell suspensions and to separate viable cells depending on their different characteristics - such as size, density, surface charge and immunological identity. Each chapter offers direct practical guidance for the various techniques and describes the advantages and limitations of each. Reproducible protocols, hints and tips for success and troubleshooting suggestions are provided.


Contents

1. Preparation of single cell suspensions; 2. Fractionation of cells by sedimentation methods; 3. Centrifugal elutriation; Appendix: Simulation of centrifugal elutriation for the Beckman E-6B chamber; 4. Separation and frustrationation of cells by partitioning in aqueous two-phase systems; Appendix: Manually operated counter current distribution apparatus; 5. Separation cells by flow cytometry; 6. Immuno methods: magnetic, columns and panning techniques; 7. Separation of cells using free flow electrophoresis; Appendix: Suppliers

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