Books  Animal & General Biology  Biochemistry & Molecular Biology 

Laboratory Protocols for Conditional Gene Targeting

Handbook / Manual

By: Raul M Torres and Ralf Kuhn

Oxford University Press

Hardback | Jul 1997 | #83572 | ISBN: 019963677X
Availability: Usually dispatched within 2-3 weeks Details
NHBS Price: £130.00 $166/€155 approx

About this book

Conditional gene targeting is an emerging technique for introducing mutations into the genes of laboratory mice. This collection of protocols comes from one of the world's premier laboratories for gene targeting The Institute of Genetics in Cologne and is intended for newcomers to the field as well as more experienced researchers. Topics covered include: the Cre/loxP recombination system and gene targeting in ES cells; gene targeting strategies; Cre/loxP recombination system; Gene targeting vectors consideration and use of loxP; nonselectable modifications and removal of selection marker genes; large deletions; gene replacement; chromosomal translocations; targeted integrations; conditional gene modification; LoxP-containing transgenes; conditional gene targeting; working with ES cells; ES cell transfection overview; embryonic feeder cells; ES cells handling and use; ES cell culture and transfection; ES clone picking; identification of homologous recombinants; freezing ES clones; thawing ES clones; genomic DNA preparation; cre-mediated neomycin deletion in ES cells; generation of mice preparation; mouse breeding for ES cell injection or aggregation; injection needles and holders; blastocyst preparation/injection; embryo aggregation; blastocyst transfer;.


Contents

Section 1: The Cre-loxP recombination system and gene targeting in ES cells; 1. Gene targeting strategies; 2. Cre-loxP recombination system; 3. Gene targeting vectors - consideration and use of loxP; 4. Nonselectablae modifications and removal of selection marker genes; 5. Large deletions; 6. Gene replacement; 7. Targeted integrations; 9. Conditional gene modification; 10. LoxP-containing transgenes; 11. Conditional gene targeting; Section 2: Working with ES cells; 12. ES cell transfection overview; 13. Embryonic feeder cells; 14. ES cells - handling and use; 15. ES cell culture and transfection; 16. ES clone picking; 17. Identification of homologous recombinants; 18. Freezing ES clones; 19. Thawing ES clones; 20. Genomic DNA preparation; 21. Cre-mediated neomycin deletion in ES cells; 22. Generation of mice - preparation; 23. Mouse breeding for ES cell injection or aggregation; 24. Injection needles and holders; 25. Blastocyst preparation/injection; 26. Embryo aggregation; 27. Blastocyst transfer; Appendices; References; Selected references for further reading

Write a review

There are currently no reviews for this product. Be the first to review this product!

Bestsellers in this subject

Ancestral Sequence Reconstruction

NHBS Price: £89.99 $115/€107 approx

The Cell Cycle

NHBS Price: £34.99 $45/€42 approx

The Molecular Life of Plants

NHBS Price: £47.50 $60/€57 approx

The Cyanobacteria

NHBS Price: £215.00 $274/€257 approx

Molecular Evolution

NHBS Price: £41.99 $53/€50 approx