Books  Animal & General Biology  Biochemistry & Molecular Biology 

RNA Processing: A Practical Approach, Volume 1

Handbook / Manual

Series: Practical Approach Series Volume: 1

Edited By: Stephen J Higgins and B David Hames

IRL Press

Paperback | Feb 1994 | #83976 | ISBN: 0199633436
Availability: Usually dispatched within 2-3 weeks Details
NHBS Price: £69.99 $86/€78 approx

About this book

Post-transcriptional events are increasingly recognized as important sites of regulation of gene expression in eukaryotic cells and so RNA processing has rapidly become an area of intense research activity. These two volumes give detailed practical guidance on all the major aspects of this subject. Step-by-step practical protocols from leading laboratories are presented for studies of the termination and end-processing, capping, methylation, splicing, and editing of mRNA as well as studies of mRNA stability and processing of rRNA and tRNA. The reader is led through all the key steps required for a successful experimental investigation. This includes full descriptions of the synthesis and purification of RNA substrates for in vitro work, the characterization of specific RNAs, and the isolation and analysis of ribonucleoprotein complexes. RNA processing - A Practical Approach, Volumes I and II, are invaluable laboratory companions for researchers working on mRNA, rRNA, or tRNA gene expression. Their lucid explanatory style and comprehensive coverage of all the key techniques for analysing RNA processing will be welcomed by both experienced researchers and

"The author's objectives have been well met in these books, which contain very concise and detailed descriptions of current and pertinent protocols for RNA processing....These technical guidebooks would be of benefit for anyone in research who deals with the molecular biology of RNA. The authors have brought together a fine group of contributors for this book who are highly qualified and authoritative.....Well written by a number of experienced authorities....This set should be in all university and community libraries, and the industrial research field should also find a great deal of use from this set as well. It should also be made available to individuals through university bookstores." --Mark A. Jandreski, PhD, Loyola University Medical Center, Doody's Journal
"Useful manuals for laboratory technicians looking for practical hints in RNA processing. I highly recommend them as laboratory methods guides for laboratories involved with RNA research and as useful references for t


1. Synthesis and purification of RNA substrates; 2. Characterization of RNA; 3. Splicing of mRNA precursors in mammalian cells; 4. Isolation and characterization of ribonucleoprotein complexes; 5. Analysis of ribonucleoprotein interactions; 6. Analysis of pre-mRNA splicing in yeast; Appendix

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