Books  Animal & General Biology  Biochemistry & Molecular Biology 

RNA Processing: A Practical Approach, Volume 2

Handbook / ManualOut of Print

Edited By: Stephen J Higgins and B David Hames

IRL Press

Paperback | Feb 1994 | #83977 | ISBN: 019963470X
Out of Print Details

About this book

Post-transcriptional events are increasingly recognized as important sites of regulation of gene expression in eukaryotic cells and so RNA processing has rapidly become an area of intense research activity. These two volumes give detailed practical guidance on all the major aspects of this subject. Step-by-step practical protocols from leading laboratories are presented for studies of the termination and end processing, capping, methylation, splicing and editing of mRNA as well as studies of mRNA stability and processing of rRNA and tRNA. The reader is led through all key steps required for successful experimental investigation. This includes full desciptions of the synthesis and purification of RNA substrates for in vitro work, the characterization of specific RNA's and the isolation and analysis of ribonucleoprotein complexes.


Contents

1. 3'-end processing of mRNA; 2. Capping and methylation of mRNA; 3. RNA editing in mitochondria; 4. Analysis of messenger RNA turnover in cell-free extracts from mammalian cells; 5. Ribosomal RNA processing in vertebrates; 6. Processing of transfer RNA precursors; 7. Ribozymes; Appendix

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