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About this book
Research on ion channels now crosses most traditional subject boundaries and involves a wide range of approaches including some of the latest techniques in biochemistry and molecular biology, as well as advanced electrophysiology and biophysics. This volume describes up-to-date practical information for some of the most essential and exciting approaches currently available. This book is intended for postgraduates, postdoctural researchers and other academic staff in the biological and biomedical sciences including biochemistry, physiology, pharmacology, and molecular biology. Also workers in the pharmaceutical industry.
Contents
1. Patch-clamp recording; 2. The measurement of the function of ion channels reconstituted into artifical membranes; 3. Concepts of single-channel analysis: inferring function from fluctuations; 4. Assays for ion channels: design and practice; 5. Channel cloning, mutagenesis and expression; 6. Antibodies as tools for the study of the structure and function of channel proteins; 7. Assembly, post-translational processing and localization of ion channels; 8. Ion channels and signal transduction systems in plant and animal cells; 9. Channel structure; 10. Electron microscope analysis of ion channels
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