Chromatography of Mycotoxins

About this book

This work comprises two parts: Part A - Techniques, and Part B - Applications. In Part A the most important principles of sample preparation, extraction, clean-up, and of established and prospective chromatographic techniques are discussed in relation to mycotoxins. In Part B the most important data, scattered in the literature, on thin-layer, liquid, and gas chromatography of mycotoxins have been compiled. Mycotoxins are mostly arranged according to families, such as aflatoxins, trichothecenes, lactones, and so on. Chromatography of individual important mycotoxins and multi-mycotoxin chromatographic analyses are also included. Applications are presented in three chapters devoted to thin-layer, liquid, and gas chromatography of mycotoxins.

Contents

Part A Techniques: 1. Sampling, Sample Preparation, Extraction and Clean-up, V. Betina: Sampling and Sample Preparation; Sample Extraction and Clean-up; Illustrative Example. 2. Techniques of Thin Layer Chromatography, R.D. Coker et al: Clean-up Methods; Normal Phase TLC; Reverse-phase TLC (RPTLC); High Performance Thin Layer Chromatography (HPTLC); Preparative TLC; Detection; Quantitative and Semi-Quantitative Evaluation; Illustrative example. 3. Techniques of Liquid Column Chromatography, P. Kuronen: Sample Pretreatment; Column Chromatography; Mini-Column Chromatography; High-Performance Liquid Chromatography. 4. Techniques of Gas Chromatography, R.W. Beaver: Resolution in Gas Chromatography; Extracolumn Resolution. 5. Emerging Techniques: Immunoaffinity Chromatography, A.A.G. Candlish and W. H. Stimson; Immunoaffinity Chromatography Theory; Practical Aspects and Instrumentation; Sample Preparation; Illustrative Examples. 6. Emerging Techniques - Enzyme-Linked lmmunosorbent Assay (ELISA) as Alternatives to Chromatographic Methods: C. M. Ward et al: Principles of ELISA; Sample Preparation; Instrumentation and Practice; Illustrative Examples. Part B Applications: 7. Thin-Layer Chromatography of Mycotoxins, V. Betina: Aflatoxins; Sterigmatocystin and Related Compounds; Trichothecenes; Small Lactones; Macrocyclic Lactones; Ochratoxins; Rubratoxins; Hydroxyanthraquinones; Epipolythiopiperazine-3,6-diones; Tremorgenic Mycotoxins; Alternaria Toxins; Citrinin; G-Cyclopiazonic Acid; PR Toxin and Roquefortine; Xanthomegnin, Viomellein and Vioxanthin; Naphtho-y-Pyrones; Secalonic Acids; TLC of Miscellaneous Toxins; Multi-Mycotoxin TLC; TLC in Chemotaxonomic Studies of Toxigenic Fungi. 8. Liquid Column Chromatography of Mycotoxins, J.C. Frisvad and U. Thrane: Column Chromatography; Mini-Column Chromatography; High Performance Liquid Chromatography; Informative On-line Detection Methods. 9. Gas Chromatography of Mycotoxins, P.M. Scott: Trichothecenes; Zearalenone; Moniliformin; Alternaria Toxins; Slaframine and Swainsonine; Patulin; Penicillic Acid; Sterigmatocystin; Aflatoxins; Ergot Alkaloids; Miscellaneous Mycotoxins.

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