Current Practice in Liquid Chromatography - Mass Spectrometry

About this book

Combined liquid chromatography-mass spectrometry has a long history of promises and breakthroughs. Many interfaces have been developed and commercialized over the past 25 years. Most of these have subsequently disappeared again, because of apparent problems, e.g. moving belt, direct liquid introduction, and thermospray. In the past few years, a real breakthrough has been made and years of promises are redeemed. Interfaces applied in combination with atmospheric-pressure chemical ionization have changed LC-MS, especially with respect to ease of operation, robustness, detection limits, and applicability ranges. LC-MS and related techniques have entered routine laboratories within pharmaceutical industries and related contract research institutes, laboratories concerned with biochemistry, biotechnology, environmental analysis, natural product research, and many other areas. Furthermore, other mass analysers than linear (triple) quadrupole instruments have found extensive use. From this perspective, the editors have invited authors both from fundamental, innovative instrumental and application-oriented research groups to contribute papers to this issue on Current Practice of LC-MS. The result is a clear perspective on the current practice of LC-MS, as well as on new instrumental developments taking place.

Contents

Pharmaceutical and Biomedical Applications of LC-MS . Automated analytical/preparative high-performance liquid chromatography-mass spectrometry system for the rapid characterization and purification of compound libraries (L. Zeng et al .). Application of liquid chromatography-mass spectrometry analyses to the characterization of novel glyburide metabolites formed in vitro (P.R. Tiller et al .). Use of liquid chromatography-tandem mass spectrometry for the quantitative and qualitative analysis of an antipsychotic agent and its metabolites in human plasma and urine (G.J. Dear et al .). Immunoaffinity chromatography combined on-line with high-performance liquid chromatography-mass spectrometry for the determination of corticosteroids (C.S. Creaser et al .). Enantioselelctive determination of terazosin in human plasma by normal phase high-performance liquid chromatography-electrospray mass spectrometry (A.P. Zavitsanos et al .). Quantitative determination of ceftiofur in milk by liquid chromatography-electrospray ionization mass spectrometry (G.K. Poon). Analysis of catechol-type glucuronides in urine samples by liquid chromatography-electrospray ionization-tandem mass spectrometry (H. Keski-Hynnila et al .). Quantitative analysis of methionine enkephalin and beta-endorphin in the pituitary by liquid secondary ion mass spectrometry and tandem mass spectrometry (Review) (D.N. Desuderuim, X, Zhu). Analysis of DNA adducts by capillary methods coupled to mass spectrometry: a perspective (Review) (W.A. Apruzzese, P. Vouros). Liquid chromatography-mass spectrometry in nucleoside, nucleotide and modified nucleotide characterization (Review) (E.L. Esmans et al .). Environmental Applications of LC-MS . Investigation of photochemical behavior of pesticides in a photolysis reactor coupled on-line with a liquid chromatography-electrospray ionization tandem mass spectrometry system. Application to trace and confirmatory analyses in food samples (D.A. Volmer). Comparison of automated on-line solid-phase extraction followed by liquid chromatography-mass spectrometry with atmospheric pressure chemical ionization and particle beam mass spectrometry for the determination of a priority group of pesticides in environmental waters (C. Aguilar et al .). Characterization of surfactants and their biointermediates by liquid chromatography-mass spectrometry (Review) (A. Di Corcia). Selective analysis of the herbicides glyphosate and aminomethylphosphonic acid in water by on-line solid-phase extraction-high-performance liquid chromatography-electrospray ionization mass spectrometry (R.J. Vreeken et al .). Rapid target analysis of microcontaminants in water by on-line single-short column liquid chromatography combined with atmospheric pressure chemical ionization ion-trap mass spectrometry (A.C. Hogenboom et al .)

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