2136 pages, colour & b/w photos, illustrations, tables
Having identified a gene product, how do you determine what it does? This manual seeks to answer this question, with a collection of strategies and techniques for the study of cellular components. It contains step-by-step protocols, ranging widely from basic culture of vertebrate and non-vertebrate cells, through advanced detection methods using immunological, cytochemical and molecular in-situ approaches, and imaging techniques such as confocal and deconvolution microscopy.
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