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Genome Analysis Laboratory Manual, Volume 1: Analyzing DNA

Handbook / ManualOut of Print

Series: Genome Analysis Laboratory Manual Series Volume: 1

Edited By: Bruce Birren, Eric D Green, Sue Klapholz, Richard M Myers and Jane Roskams

675 pages, Illus

Cold Spring Harbor Laboratory Press

Spiralbound | Oct 1997 | #85457 | ISBN: 0879694963
Out of Print Details
Hardback | Dec 1997 | #85456 | ISBN: 0879694955
Out of Print Details

About this book

Assuming only a basic knowledge of molecular biology, this is the first in a series of manuals which explains how to clone, manipulate, analyze and sequence large segments of DNA, and relate expressed sequence to phenotypic variation. The techniques are written for application to animal DNA as well as human genomes. They deal plainly with sources of failure, and solutions.


Contents

Purifying and analyzing genomic DNA; preparation, manipulation and mapping of HMW DNA; PCR in genome analysis; fluorescence-based DNA sequencing; shotgun sequencing; transposon-mediated DNA sequencing; computational analysis of DNA and protein sequences. Appendices: common reagents; basic procedures; safety precautions; useful facts; suppliers.

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