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About this book
Assuming only a basic knowledge of molecular biology, this is the first in a series of manuals which explains how to clone, manipulate, analyze and sequence large segments of DNA, and relate expressed sequence to phenotypic variation. The techniques are written for application to animal DNA as well as human genomes. They deal plainly with sources of failure, and solutions.
Contents
Purifying and analyzing genomic DNA; preparation, manipulation and mapping of HMW DNA; PCR in genome analysis; fluorescence-based DNA sequencing; shotgun sequencing; transposon-mediated DNA sequencing; computational analysis of DNA and protein sequences. Appendices: common reagents; basic procedures; safety precautions; useful facts; suppliers.
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