Enzyme and Microbial Biosensors Techniques and Protocols

About this book

This is a cutting-edge collection of detailed, step-by-step techniques and protocols for constructing, evaluating, and using enzyme- and microbial-based biosensors. Ideal for novices starting research in their field or experienced researchers wanting to use a biosensor for a specific analytical measurement, the methods detailed here allow biochemists, analytical chemists, microbiologists, and engineers to successfully apply biosensor technology to their specific problems. The techniques include classical enzyme biosensors based on potentiometric, amperometric, conductimetric, optical, and thermal transducers, as well cell-based biosensors based on amperometric and optical transducers. A companion volume, "Affinity Biosensors: Protocols and Techniques", by Rogers & Mulchandani, concentrates on cell- and affinity-based biosensors.

Contents

Part I.Enzyme Biosensors. Principles of Enzyme Biosensors Ashok Mulchandani. Enzyme Biosensors Based on pH Electrode Canh Tran-Minh . Enzyme Biosensors Based on Gas Electrodes Marco Mascini and Gianna Marrazza. Enzyme Biosensors Based on ISFETs Roland Ulber and Thomas Scheper. Enzyme Biosensors Based on Oxygen Detection F. W. Scheller D. Pfeiffer F. Lisdat C. Bauer and N. Gajovic. Enzyme Biosensors Based on the Hydrogen Peroxide Electrode John Woodward. Enzyme Biosensors Based on Mediator-Modified Carbon Paste Electrode Prem C. Pandey. Enzyme Biosensors Based on Electron Transfer Between Electrode and Immobilized Peroxidases Lo Gorton Elisabeth Csregi Tautgirdas Ruzgas Irina Gazaryan and Gyrgy Marko-Varga. Enzyme Biosensors Based on Redox Polymers Latha Shankar Michael G. Garguilo and Adrian C. Michael . Enzyme Biosensors Based on Metallized Carbon Electrodes Joseph Wang. Enzyme Biosensors Based on Conducting Polymers Wolfgang Schuhmann. Enzyme Sensors Based on Conductimetric Measurement Norman F. Sheppard Jr. and Anthony Guiseppi-Elie. Enzyme Biosensors Based on Thermal Transducer/Thermistor Kumaran Ramanathan Masoud Khayyami and Bengt Danielsson. Enzyme Biosensors Based on Fluorometric Detection Ashutosh Sharma. Part II. Microbial Biosensors. Microbial Biosensors Based on Oxygen ElectrodesKlaus Riedel . Microbial Biosensors Based on Respiratory Inhibition Yoshiko Arikawa Kazunori Ikebukuro and Isao Karube. Microbial BiosensorsBased on Potentiometric Detection Aleksandr L. Simonian Evgenia I. Rainina and James R. Wild. Microbial Biosensors Based on Optical Detection Udayakumar Matrubutham and Gary S. Sayler. Index .

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