A comprehensive collection of readily reproducible methods for studying receptors in silico, in vitro, and in vivo. These cutting-edge techniques cover mining from curated databases, identifying novel receptors by high throughput screening, molecular methods to identify mRNA encoding receptors, radioligand binding assays and their analysis, quantitative autoradiography, and imaging receptors by positron emission tomography (PET). Highlights include phenotypic characterization of receptors in knockout mice, imaging receptors using green fluorescent protein and fluorescent resonance energy transfer, and quantitative analysis of receptor mRNA by TaqMan PCR. These book equips the researcher with techniques for exploring the unprecedented number of new receptor systems now emerging and the so-called "orphan" receptors whose activating ligand has not been identified.
Research scientists who study or wish to study drug/hormone actions would benefit from this book. It is applicable for students, instructors, and advanced scientists. The editor has successfully compiled this book for the intended readers...I find this book a very useful and practical addition to the field of receptor pharmacology...This book should be open and on the bench of every student of receptor pharmacology. - Doody's Health Sciences Book Review Journal step-by-step experimental protocols demonstrate the power of the radioligand binding an extremely useful book. - Cellular and Molecular Biology found the book easy to read and full of useful information. Having been involved in receptorology during the past 16 years, I found the content of this book comprehensive and up to date and as such the book will be useful for both the specialist as well as one just beginning in the area. - Cell Biology International ...does much to dispel the mysteries surrounding the technique and provides a good many solutions to practical problems...The book is written in a uniformly accessible style and there is plenty of benefit to be derived from it for both novices and more experiences scientists wanting to learn a new technique. - Microbiology Today
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