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Methods in Plant Molecular Biology

Handbook / ManualOut of Print

By: M Schuler

Academic Press

Hardback | Dec 1988 | #89146 | ISBN: 0126323402
Out of Print Details

About this book

A laboratory manual introducing students to a diversity of molecular techniques needed for experiments with plant cells. The manual integrates protein, RNA and DNA techniques, and the handling of recombinant DNA or purified proteins.

It is hard to tell who will benefit most from this compilation, the students or the faculty. The selection of experiments should satisfy the cravings of students to learn the most prominent techniques of molecular biology. The experimental detail and organization of the book are superb and should provide any faculty grappling with course work in plant molecular biology a top notch, turnkey laboratory course. The manual will be equally valuable to postdoctoral students who are entering the plant field because of the wide applicability of the techniques described. This volume will be a valuable addition to all plant science faculties. --Arthur Weissbach, Roche Institute of Molecular Biology as seen in ANALYTICAL BIOCHEMISTRY "This book is a very fine manual for a one semester laboratory course demonstrating basic methods of molecular biology as they apply to the use of higher plants as the research subject. Methods in Plant Molecular Biology serves very well as a laboratory manuel for an introductory course in plant molecular biology or as a source of supplementary plant experiments for a general molecular biology course." --PLANT GENETICS NEWSLETTER "This course will give students and researchers a valuable introduction to the latest techniques in plant molecular biology." --PLANT BREEDING ABSTRACTS


Contents

Preface. Laboratory Schedule. Restriction Mapping of Plasmid DNA. Cloning of Restriction Fragments into pUC Vectors. Preparation of Intact Chloroplasts from Pea. Lowry Assay for Protein Determination. Protein Synthesis by Isolated Pea Chloroplasts. Separation of Thylakoid and Stomal Proteins by SDS-Gel Electrophoresis. Isolation of Chloroplast DNA. RNA Isolation from Light- and Dark-Grown Seedlings. Preparation of a Wheat Germ Extract for in Vitro Translation of mRNA. Screening of Recombinant Phage Libraries with Cloned cDNA Probes. Isolation of Phage DNA from Liquid Cultures. Dideoxy DNA Sequencing. Transformation of Leaf Discs with Agrobacterium. Glossary. Gel Electrophoresis Equipment. Index.

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