Books  Animal & General Biology  Biochemistry & Molecular Biology 

High Resolution Chromatography

Handbook / Manual

Edited By: Paul Millner

320 pages, 5 b/w illus, 70 figs

Oxford University Press

Paperback | Apr 1999 | #90275 | ISBN: 0199636486
Availability: Usually dispatched within 6 days Details
NHBS Price: £69.99 $89/€84 approx
Hardback | Apr 1999 | #90274 | ISBN: 0199636494
Availability: Usually dispatched within 2-3 weeks Details
NHBS Price: £130.00 $165/€155 approx

About this book

The molecular biology revolution has required the development of new chromatographic techniques and the optimization of original techniques to give reasonable quantities of protein at high resolutions. The aim of this volume is to provide the necessary information in most experimental situations to enable rapid and effective purification. The first four chapters deal with the instrumental aspects of high resolution chromatography starting with the initial clean up steps prior to separation in chapter 1. Chapter 2 deals with microscale techniques, then chapter 3 describes the detector technologies that can determine information about the separated molecules. The final chapter in this section cover capillary electrophoresis and its associated techniques. The remaining chapters cover a range of chromatographic procedures based on the interaction of a specific ligand with its target protein or other macromolecule. Some chapters cover non-specific interactions using peptides, inhibitors, and antibodies as the affinity ligand while others focus on specific groups of molecules : oligosaccharides and glycosylated proteins, nucleotide-binding proteins, proteins binding free and chelated metal ions, and DNA binding proteins.


Contents

Separation by charge, size and hydrophobicity; Practice and benefits of microcolumn HPLC; Detection devices; Capillary electrophoresis of peptides and proteins; Lectins as affinity probes; Nucleotide-and dye-ligand chromatography; Synthetic peptides as affinity ligands; Drugs and inhibitors as affinity ligands; Immunoaffinity chromatography; Strategies and methods for purification of Ca2+ -binding proteins; Purification of DNA-binding proteins

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