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DNA Cloning: A Practical Approach, Volume 1: Core Techniques

Handbook / Manual

Series: DNA Cloning: A Practical Approach Volume: 1

Edited By: BD Hames and D Glover

280 pages, 8 b/w photos, 32 illus

IRL Press

Paperback | May 1995 | Edition: 2 | #38561 | ISBN: 0199634769
Availability: Usually dispatched within 2-3 weeks Details
NHBS Price: £70.00 $86/€78 approx

About this book

This volume provides detailed laboratory protocols, advice, hints and tips, example data, key literature citations, background information, and troubleshooting comments for the steps used in most molecular biology laboratories. It covers transformation, construction of cDNA and genomic libraries, probe preparation, analysis of gene organisation and expression, in vitro mutagenesis, and DNA sequencing. The information provided is a completely up to date account of each topic by established researchers. These two volumes, the first of four, are a thoroughly revised and up dated version of the original ones published in the Practial Approach series. Together they will offer a complete guide to the major techniques required by modern molecular biology laboratories.


1. Techniques for transformation of E.coli; 2. Lambda replacement vectors in the construction of genomic DNA libraries; 3. Procedures for cDNA cloning; 4. Making probes; 5. Using cloned DNAs to analyse gene organization and expression; 6. Choices of single-stranded vectors and their application for in vitro mutagenesis; 7. PCR and its applications; 8. DNA sequencing

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