Books  Animal & General Biology  Genetics 

DNA Cloning: A Practical Approach, Volume 2: Expression Systems

Handbook / Manual

Series: DNA Cloning: A Practical Approach Volume: 2

Edited By: BD Hames and D Glover

253 pages, 20 b/w photos, 38 illus

IRL Press

Paperback | May 1995 | Edition: 2 | #38566 | ISBN: 0199634785
Availability: Usually dispatched within 2-3 weeks Details
NHBS Price: £70.00 $88/€83 approx

About this book

Expression systems are in widespread use for cloning specific genes, for synthesising the encoded proteins for structural and functional analysis, and for large-scale preparation of current use with background information and advice on the merits of each, step-by-step practical protocols, troubleshooting, and details of the latest applications. These two books, the first of four volumes are thoroughly revised and up-dated versions of the original ones published in the Practical Approach series. Together they will offer a complete guide to the major techniques required by modern molecularbiology laboratories.


Contents

1. Screening expression libraries with antibody and protein probes; 2. Expression of foreign proteins in E.coli using plasmid vectors and purification of specific polyclonal antibodies; 3. Purification of over-produced proteins from E.coli cells; 4. Production of monoclonal antibodies against proteins expressed in E.coli; 5. Expression of cloned genes in yeast; 6. Intraction trap cloning with yeast; 7. The baculovirus expression system

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