Books  Animal & General Biology  Biochemistry & Molecular Biology 

Imaging Living Cells

Handbook / Manual

Edited By: R Rizzuto and C Fasolato

300 pages, 97 figs, 10 tabs


Ringbound | Dec 1999 | #96002 | ISBN: 3540650512
Availability: Usually dispatched within 1-2 weeks Details
NHBS Price: £100.00 $122/€112 approx

About this book

Improved technology for imaging living cells, specific cellular targets and organelles is having a dramatic impact on basic and applied research. By combining optical design and molecular genetics, a new series of tools is being developed and successfully applied together with classical probes. Novel labelling strategies, better software for image enhancement and analysis are now available and allow image acquisition with greater speed and precision. This lab manual, intended as for bench-top use, is suitable for both scientists and graduate students, combines an update on the most advanced imaging procedures with detailed protocols. Examples, cleverly selected from the wide repertoire of cell pyhsiology, cover different functional aspects such as distribution of multiple ions, electrical activity, exo-endocytosis, gene expression, and the cell cycle.


Strategies for Quantitative Digital Imaging of Biological Activity with Ion-Sensitive Fluorescent Probes * High Resolution 3-D Imaging by Image Restoration * Imaging Calcium in the Cytoplasm and in Organelles with Fluorescent Dyes * Confocal Calcium Imaging * Imaging Whole Organs * Simultaneous Measurement of Membrane Current and Intracellular Calcium * Quantitative Calcium Imaging in Brain Slices * Common Methods for Measuring Apoptotic Cell Death with Flow Cytometry * Expressing, Targeting, and Calibrating Recombinant Aequorin * Analysis of Regulated Gene Expression by Microinjection and Digital Luminescence Imaging of Single Living Cells * The New Green Fluorescent Protein Mutants and Their Applications * Imaging Green Fluorescent Protein in Transgenic Plants.

Write a review

There are currently no reviews for this product. Be the first to review this product!

Bestsellers in this subject

Ancestral Sequence Reconstruction

NHBS Price: £89.99 $110/€101 approx

The Cell Cycle

NHBS Price: £34.99 $43/€39 approx

The Molecular Life of Plants

NHBS Price: £47.50 $58/€53 approx

The Cyanobacteria

NHBS Price: £215.00 $263/€241 approx