Genetic Methods for Diverse Prokaryotes

About this book

This new volume presents overviews of the very latest genetic approaches in a diverse range of prokaryotes. Divided into three sections, the topics include essential techniques for genetic analysis, case studies in which genetic methods in carefully chosen genera are described and approaches are used in the elucidation of specific phenomena. It includes up-to-date chapters on essential techniques for genetic analysis in diverse bacteria. It also covers the use of plasmids, phages and transposons and their applications to new organisms. It includes genetic methods in medically and industrially important bacteria such as Mycobacteria, Neisseria, Bacteroides, Clostridia, and spirochaetes. It provides an analysis of virulence in Helicobacter and Erwina; Genetic methods in Archae; Photosynthesis and respiration in Paracoccus and Rhodobacter; and Bacillus subtilis sporulation.

Contents

Preface. Essential Techniques for Genetic Analysis in Bacteria: J. Saunders and V.A. Saunders, Introduction of DNA into Bacteria. L. Radnedge and H. Richards, The Development of Plasmid Vectors. M.C.M. Smith and C.E.D. Ress, Exploitation of Bacteriophages and their Components. Paul Dyson, Isolation and Development of Transposons. Case Studies: T. Schwan, T. Rudel, T.F. Meyer, Genetic Methods in Neisseria. Genetic Methods in Clostridia: D.I. Young, V.J. Evans, J.R. Jeffries, K.C.B. Jennert, Z.E.V. Phillips, A. Ravagnani, and M. Young, Genetic Methods in Clostridia. P. Rosa, B. Stevenson, and K. Tilly, Genetic Methods in Borrelia and Other Spirochaetes. A. Salyers, N. Shoemaker, A. Cooper, J. D'Elia, and J. Shipman, Genetic Methods for Bacteroides Species. G.F. Hatfull, Genetic Methods in Mycobacteria. W.W. Metcalf, Genetic Analysis in the Domain Archaea. Genetic Approaches to Analyze Specific Complex Phenomena: E. Allan, S. Foynes, N. Dorrell, and B. Wren, Genetic Characterization of the Gastric Pathogen Helicobacter Pylori. N.R. Thomson, J.D. Thomas, and G.P. Salmond, Virulence Determinants in the Bacterial Phytopathogen Erwinia. M.D. Page and R.E. Sockett, Molecular Genetic Methods in Paracoccus and Rhodobacter with Reference to the Analysis of Photosynthesis and Respiration. D.H. Green and S. Cutting, Sporulation in Bacillus Subtilis. Subject Index.

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