About this book
This volume is an authoritative and comprehensive treatment of the approaches and techniques used for Green Fluorescent Proteins (GFP). The primary focus of this work is on research using biological systems. The volume covers all aspects of GFP, from its expression in different organisms to specific microscopic and data analysis methods.
Contents
F.G. Prendergast, Biophysics of Green Fluorescent Protein. A.B. Cubitt, L.A. Woollenweber, and Roger Heim, Understanding Structure-Function Relationships in the Aequorea victoria Green Fluorescent Protein. D.W. Piston, G.H. Patterson, and Susan M. Knobel, Quantitative Imaging of the GFP. D. Pierce and R.D. Vale, Single-Molecule Fluorescence Detection of GFP and Application to Single-Protein Dynamics. F. De Giorgi, Z. Ahmed, C. Bastianutto, M. Brini, L.S. Jouaville, R. Marsault, M. Murgia, P. Pinton, T. Pozzan, and R. Rizzuto, Targeting GFP to Organelles. J.L. Carminati and T. Stearns, Cytoskeletal Dynamics in Yeast. P. Ferrigno and P.A. Silver, Analysis of Nuclear Transport in Vivo. K.E. Sawin, GFP Fusion Proteins as Probes for Cytology in Fission Yeast. J. Haseloff, GFP Variants for Multispectral Imaging of Living Cells. S.A. Endow, GFP Fusions to a Microtubule Motor Protein to Visualize Meiotic and Mitotic Spindle Dynamics in Drosophila. A. Brand, GFP as a Cell and Developmental Marker in the Drosophila Nervous System. K.F. Sullivan and Richard D. Shelby, Using Time-Lapse Confocal Microscopy for Analysis of Centromere Dynamics in Human Cells. A.S. Belmont, G. Li, G. Sudlow, and C. Robinett, Visualization of Large-Scale Chromatin Structure and Dynamics Using the lac Operator/lac Repressor Reporter System. A. Young, R. Tuft, W. Carrington, and S. Doxsey, Centrosome Dynamics in Living Cells. G.A. Dabiri, K.K. Turnacioglu, J.C. Ayoob, J.M. Sanger, and J.W. Sanger, Transfections of Primary Muscle Cell Cultures with Plasmids Coding for GFP Linked to Full-Length and Truncated Muscle Proteins. J. Lippincott-Schwartz, J.F. Presley, K.J.M. Zaal, K. Hirschberg, C.D. Miller, and J. Ellenberg, Monitoring the Dynamics and Mobility of Membrane Proteins Tagged with GFP. J.D. Plautz and S.A. Kay, Synchronous Real-Time Reporting of Multiple Cellular Events. A. Periasamy and R.N. Daly, Visualizing Protein Interact
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Biography
Edited by Leslie I. Wilson, Paul T. Matsudaira, Kevin F. Sullivan, and SteveA. Kay