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Methods in Enzymology: Volume 302: Green Fluorescent Protein

Handbook / ManualOut of Print

Series: Methods in Enzymology Volume: 302

Edited By: Michael Conn

Academic Press

Hardback | Dec 1999 | #101367 | ISBN: 0121822036
Out of Print Details

About this book

One of the most important recent discoveries in science is the green fluorescent protein, isolated from a bioluminescent jellyfish. The gene that codes for this protein is active in any type of cell, from microbes to humans, producing a "day-glo" dye visible as a bright green light in the microscope. The power of the gene is that by using recombinant DNA technology. GFP can be spliced to any protein, essentially painting that protein green. When such a spliced protein is inside a cell. Further, time-lapse microscopy can be used to examine how that protein and the cellular components that it is part of move within the cell during the cell's life cycle. "This Methods in Enzymology" volume deals with the utility of green fluorescent protein (GFP). The OVID database (including MEDLINE, Current Contents, and other sources) lists nine references to GFP for the ten-year period 1985-1994. In contrast, in less than four years thereafter, over 500 references are listed, a testament to the rapid growth of interest in this probe. This volume documents many diverse uses for this interesting molecule in disciplines that broadly span biology. The methods presented include shortcuts and conveniences not included in previously published sources. The techniques are described in a context that allows comparisons to other related methodologies-such comparisons are valuable to readers who must adapt existing procedures to new systems. Also, so far as possible, methodologies have been presented in a manner that stresses their general applicability and potential limitations. The volume provides a substantial and current overview of the extant methodology in the field and a view of its rapid development. The critically acclaimed laboratory standard for more than forty years, "Methods in Enzymology" is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. Now, with more than 300 volumes (all of them still in print), the series contains much material still relevant today - truly an essential publication for researchers in all fields of life sciences. Key features include: Monitoring of Physiological Processes; Localization of Molecules; Special Uses; Mutants; and Variants of GFP.

Praise for the Series "The Methods in Enzymology series represents the gold-standard." --NEUROSCIENCE "Incomparably useful." --ANALYTICAL BIOCHEMISTRY "It is a true 'methods' series, including almost every detail from basic theory to sources of equipment and reagents, with timely documentation provided on each page." --BIO/TECHNOLOGY "The series has been following the growing, changing and creation of new areas of science. It should be on the shelves of all libraries in the world as a whole collection." --CHEMISTRY IN INDUSTRY "The appearance of another volume in that excellent series, Methods in Enzymology, is always a cause for appreciation for those who wish to successfully carry out a particular technique or prepare an enzyme or metabolic intermediate without the tiresome prospect of searching through unfamiliar literature and perhaps selecting an unproven method which is not easily reproduced." --AMERICAN SOCIETY OF MICROBIOLOGY NEWS "If we had some way to find the work most often consulted in the laboratory, it could well be the multi-volume series Methods in Enzymology...a great work." --ENZYMOLOGIA "A series that has established itself as a definitive reference for biochemists." --JOURNAL OF CHROMATOGRAPHY


Monitoring of Physiological Processes: S. Kupzig, S. San Lee and G. Banting, Membrane Trafficking. C. Kaether and H.-H. Gerdes, Monitoring of Protein Secretion with Green Fluorescent Protein. R.M. Hoffman, Green Fluorescent Protein to Visualize Cancer Progression and Metastasis. X. Zhao, T. Duong, C.-C. Huang, S.R. Kain and X. Li, Comparison of Enhanced Green Fluorescent Protein and Its Destabilized Form as Transcription Reporters. S.R. Kain and J.-T. Ma, Early Detection of Apoptosis with Annexin V-Enhanced Green Fluorescent Protein. M. Maniak, Green Fluorescent Protein in the Visualization of Particle Uptake and Fluid-Phase Endocytosis. F. Hanakam and G. Gerisch, Monitoring Intracellular Shuttling of Histidine-rich pH-Sensor Proteins Tagged with Green Fluorescent Protein. S.R. Cronin and R.Y. Hampton, Measuring Protein Degradation with Green Fluorescent Protein. G.L. Hager, Studying Nuclear Receptors with Green Fluorescent Protein Fusions. Localization of Molecules: M.R. Flory and T.N. Davis Localization of Calmodulin in Budding Yeast and Fission Yeast Using Green Fluorescent Protein. K.R. Olson and J.B. Olmsted, Analysis of Microtubule Organization and Dynamics in Living Cells Using Green Fluorescent Protein-MAP4 Chimeras. V. Georget, B. Terouanne, J.-C. Nicholas and C. Sultan, Trafficking of the Androgen Receptor. C.D. Webb and O. Resnekov, Use of Green Fluorescent Protein for Visualization of Cell-Specific Gene Expression and Subcellular Protein Localization in Bacillus subtilis. L.S. Barak, J. Zhang, S.S.G. Ferguson and M.G. Caron, Signaling, Desensitization, and Trafficking of G Protein Coupled Receptors Revealed by Green Fluorescent Protein Conjugates. G.A. Dabiri, J.C. Ayoob, K.K. Turnacioglu, J.M. Sanger and J.W. Sanger, Use of Green Fluorescent Proteins Linked to Cytoskeletal Proteins to Analyze Myofibrillogenesis in Living Cells. Special Uses: L. Lybarger and R. Chervenak, Use of Fluorescent Proteins in Single and Multicolor Flow Cytometry. L. Lybarger and R. Chervanak, Flow Cytometric Analysis of Transcription: Use of Green Fluorescent Protein Variants to Control Transfection Efficiency. Y. Fang, C.-C. Huang, S.R. Kain and X. Li, Use of Co-expressed Enhanced Green Fluorescent Protein as a Marker for Identifying Transfected Cells. L.A.C. Blair, K.K. Bence and J. Marshall, The Jellyfish Green Fluorescent Protein: A Tool for Studying Ion Channels and Second Messenger Signaling in Neurons. A. Miyawaki, J.M. Matheson, L.G. Sayers, A.Muto, T. Michikawa, T. Furuichi and K. Mikoshiba, Expression of Green Fluorescent Protein and Inositol 1,4,5-Trisphosphate Receptor in Xenopus laevis Oocytes. T. Takada, K. Yoshida, K. Nakamura, G. Tsujimoto, M. Katsuki and S. Sugano, Expression of Green Fluorescent Protein in Transgenic Mice. A.S. Verkman, Green Fluorescent Protein as a Probe to Study Intracellular Solute Diffusion. T. Aoki, K.S. Koch, H.L. Leffert and H. Watabe, Application of Green Fluorescent Protein-Protein A Fusion Protein to Western Blotting. P. Prabhakara, M. Kallio and J.C. Herr, Green Fluorescent Protein As a Reporter for Promoter Analysis of Testis-Specific Genes in Transgenic Mice. L.E. Bermudez, F.J. Sangari and A. Parker, Use of Green Fluorescent Protein for the Measurement of Bacteria-Host Interactions. D.W. Galbraith, Flow Cytometric Analysis of Transgene Expression in Higher Plants: Green Fluorescent Protein. P.E. Urwin, S.G. Moller, J.K. Blumsom and H.J. Atkinson, Continual Green Fluorescent Protein Monitoring of Promoter Activity in Plants. J.P. Levy, R.R. Muldoon, C.J. Link, Jr. And S.R. Kain, Retroviral Expression of Green Fluorescent Protein. J.J. Lemasters, D.R. Trollinger, T. Qian, W.E. Cascio and H. Ohata, Confocal Imaging of Ca2+, pH, Electrical Potential and Membrane Permeability in Single Living Cells. J.P. Levy, R.R. Muldoon, I.A. Mazo, S.R. Kain and C.J. Link, Jr., In Vivo Retroviral Transduction and Expression of Green Fluorescent Protein. Mutants and Variants of Green Fluorescent Protein: Y. Kimata, C. Ren Lim and K. Kohno, S147P Green Fluorescent Protein: A Less Thermosensitive Green Fluorescent Protein Variant. G.J. Palm and A. Wlodawer, Spectral Variants of Green Fluorescent Protein. L.A. King, C.J. Thomas, N. Wilkinson and R.D. Possee, Expression of Green Fluorescent Protein Using Baculovirus Vectors. R. Heim, Green Fluorescent Protein Forms for Energy Transfer. C.J. Link, Jr., S. Wang, R.R. Muldoon, T. Seregina and J.P. Levy, Use of Codon Modified, Red-shifted Variants of Green Fluorescent Protein Genes to Study Viral Mediated Gene Transfer. X. Zhao, X. Jiang, C.-C. Huang, S.R. Kain and X. Li, Generation of a Destabilized Form of Enhanced Green Fluorescent Protein. S. Inouye, K. Umesono, and F.I. Tsuji, Spectral Properties of Green Fluorescent Protein-S65A.

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