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Molecular Methods in Developmental Biology: Xenopus and Zebrafish

Handbook / Manual

Series: Methods in Molecular Biology Volume: 123

Edited By: Matthew Guille

217 pages, Figs, tabs

Humana Press

Hardback | Dec 1999 | #104091 | ISBN: 0896037908
Availability: Usually dispatched within 1-2 weeks Details
NHBS Price: £89.50 $114/€107 approx

About this book

A collection of basic and essential molecular and embryological techniques for studying Xenopus and zebrafish. Easily reproducible and designed to succeed, these detailed methods include cellular techniques, techniques for the quantitative and spatial analysis of mRNA and proteins, and techniques for the expression of gene products in embryos.

Readers will find the systematic description of methods, illustration of structures, tables and figures, along with comments on optimizing the methods for use in other systems, very useful. this excellent book will facilitate studies on the lipidation of proteins. It should be useful to both beginning and experienced investigators in the field. - Analytical Biochemistry this compact book comprises concise and very well structured chapters on both basic and advanced molecular and embryological technique used in studies of early stages of vertebrate development on two model species, the South Africa clawed frog Xenopus loevis and the zebrafish Brachydanio rerio. All chapters have been written by authors with first-hand expertise in individual fields, mostly those in whose laboratories the methods in question have been developed, or at least optimized. Close attention is paid to meticulous description of possible pitfalls and drawbacks, and essential steps are stressed. the book contains a wealth of information, cues on methodology and their critical assessment. It is an almost indispensable guide for anybody working in the field of vertebrate development biology. - Folia Microbiologica ...this book is a solid introduction to some of the common techniques in zebrafish and Xenopus and I think would be of good use to its intended audience of new workers in the field. - Newsletter of the British Society of Developmental Biology


Contents

The Xenopus Animal Cap Assay. Cell and Tissue Transplantation in Zebrafish Embryos. Ribonuclease Protection Analysis of Gene Expression in Xenopus,. Quantitative Analysis of mRNA levels in Xenopus Embryos by Reverse Transcriptase-Polymerase Chain Reaction (RT-PCR). Whole-Mount in situ Hybridization of Xenopus and Zebrafish Embryos. In situ Hybridization to Sections of Xenopus Embryos. Zebrafish Immunohistochemistry. Xenopus Immunohistochemistry. Preparation and Testing of Synthetic mRNA for Microinjection. Microinjection into Xenopus Oocytes and Embryos. Microinjection into Zebrafish Embryos, Qiling Xu. Expression from DNA Injected into Xenopus Embryos. Promoter Analysis in Zebrafish Embryos. Transient Transgenesis in Xenopus Laevis Facilitated by AAV-ITRs. Gel Shift Analysis of DNA-Binding Proteins in Crude Oocyte and Embryo Extracts. High Resolution DNA Footprinting Techniques with Crude Oocyte and Embryo Extracts. Mapping Protein-DNA Interactions Using In Vivo Footprinting.

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