Books  Animal & General Biology  Biochemistry & Molecular Biology 

Monoclonal Antibodies

Handbook / Manual

Series: Practical Approach Series

Edited By: Philip Shepherd and Christopher Dean

576 pages, Illus

Oxford University Press

Paperback | May 2000 | #107212 | ISBN: 0199637229
Availability: Usually dispatched within 2-3 weeks Details
NHBS Price: £69.99 $88/€83 approx
Hardback | Dec 2000 | #107211 | ISBN: 0199637237
Out of Print Details

About this book

Monoclonal Antibodies: A Practical Approach covers the preparation, testing, derivation, and applications of monoclonal antibodies. New immunological techniques incorporating tried and tested methodologies are described, making the book of interest to established and inexperienced immunologists. Both the standard somatic hybridization technique and recombinant techniques, including the use of phage libraries, for the preparation of rodent and human monoclonal antibodies are described. Protocols for both the small and large scale production are detailed, as well as purification and labelling (with both radioisotopes and non-radioisotopes) methods. The applications of monoclonal antibodies in immunoblotting, enzyme linked immunoassays, immunofluorescence, and FACS analysis are all covered in detail. Finally protocols are given for the use of monoclonal antibodies in rheumatoid arthritis, tissue typing, detecting DNA modified during chemotherapy, and in the clinical analysis of transplantation samples for malignancy. This book will therefore be an invaluable laboratory companion to anyone using monoclonal antibodies in their research.

A useful and practical addition to the field of cell biology ... many research scientists, students and technicians will benefit from step-by-step experimental protocols demonstrating the power of hybridoma technologies. Cell Biology International An authoritative team of investigators brings together a series of concise and proven methods used in their laboratories and laboratories around the world, which can be used by both basic scientists working in the field of cell and molecular biology as well as clinical researchers interested in the clinical application of monoclonal antibodies. An experienced molecular biologist without much expertise with immunochemical methods and immunologists, pathologists and others will find this book very helpful. For undergraduate and graduate students this inexpensive book is a good source of information on methods, which are shown in the form of concise protocols and well referenced, and general use of monoclonal antibodies in research and clinical laboratories. Cell Biology International


Contents

Preparation of Rodent Monoclonal Antibodies by in vitro Somatic Hybridization; Preparation of Recombinant Antibodies From Immune Rodent Spleens and the Design of Their Humanisation by CDR Grafting; Selection of Antibodies From Phage Libraries of Immunoglobulin Genes; Arm Complexes for in vitro Display and Evolution of Antibody Combining Sites; Human Monoclonal Antibodies to Blood Group Antigens; Laboratory Based Methods for Small-Scale Productions of Monoclonal Antibodies; Isolation and Purification of Antibodies From Tissue Culture Supernatant; Antibody Production in Plants; Radiolabelling of Monoclonal Antibodies; Non-Radioactive Antibody Probes; Immunogold Probes for Light and Electron Microscopy; Characterization of Cellular Antigens Using Monoclonal Antibodies; Immunoassays; Immunoaffinity Chromatography of Macromolecules; Immunochemical Detection of BrdUrd-Labelled Nuclei for Monitoring Cell Kinetics; Immunofluorescence Microscopy; Analysis of Clinical Haematological Samples in Transplantation for Cancer; Immunocytochemical Staining of Cells and Tissues for Diagnostic Applications; Detection of Chemically Modified DNA in Lymphocytes of Patients Undergoing Chemotheraphy; Monoclonal Antibody Therapy in Organ Transplantation; Monoclonal Antibody Therapy in Rheumatoid Arthritis

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