Transgenic Cotton Methods and Protocols

About this book

Cotton is the most important textile and cash crop and is widely cultivated in more than 70 countries, including the United States, China, and India. Because its long life cycle and complicated genetic background, it is hard to improve cotton using traditional breeding techniques although it has made much progress in the last several decades. Currently, transgenic techniques have become a powerful tool to improve cotton and transgenic cotton is among the first commercially genetically modified crops.

Transgenic Cotton: Methods and Protocols provides a comprehensive collection of methods for creating and monitoring transgenic cotton and its application on agricultural and basic research. Divided into five convenient sections, topics covered include the current status and perspectives of transgenic cotton, the principle and methods for making transgenic cotton, the methods for detecting foreign gene copy and expression in transgenic plants, the improvement of cotton using transgenic technology, and finally the methods for monitoring the potential impact of transgenic cotton on environment, including gene flow.

Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and easily accessible, Transgenic Cotton: Methods and Protocols will serve as an excellent resource for scientists as well as graduate students who work on transgenic plants, plant genetics, molecular biology and agricultural sciences.

Contents

Part I. Introduction
1. Transgenic Cotton: From Biotransformation Methods to Agricultural Application Baohong Zhang
2. Genetically Modified Cotton in India and Detection Strategies Gurinder Jit Randhawa and Rashmi Chhabra

Part II. Transformation
3. Agrobacterium-Mediated Transformation of Cotton Baohong Zhan
4. Biolistic Transformation of Cotton Zygotic Embryo Meristem Rajah Rajasekaran
5. Biolistic Transformation of Cotton Embryogenic Cell Suspension Cultures Rajah Rajasekaran
6. Cotton Transformation via Pollen Tube Pathway Baohong Zhang and Qinglian Wang
7. Silicon Carbide Whisker-Mediated Transformation of Cotton (Gossypium hirsutum L.) Muhammad Arshad, Yusuf Zafar, and Shaheen Asad

Part III. Detection
8. Investigating Transgene Integration and Organization in Cotton (Gossypium hirsutum L.) Genome Jun Zhang and Yan Hong
9. Estimating the Copy Number of Transgenes in Transformed Cotton by Real-Time Quantitative PCR Chengxin Yi and Yan Hong
10. Development of Enzyme-Linked Immunosorbant (ELISA) Assay for the Detection of Bt Protein in Transgenic Cotton Abhishek Ojha, Arvind Kumar, and Suchitra Kamle
11. DNA-Based Diagnostics for Genetically Modified Cotton: Decaplex PCR Assay to Differentiate MON531 and MON15985 Bt Cotton Events Gurinder Jit Randhawa, Monika Singh, and Rashmi Chhabra
12. A Simple and Rapid Method for Determining Transgenic Cotton Plants Baohong Zhang, Hongmei Wang, Fang Liu, and Qinglian Wang
13. An Efficient Grafting Technique for Recovery of Transgenic Cotton Plants Min Wang, Qinglian Wang, and Baohong Zhang
14. Inheritance of Transgenes in Transgenic Bt Lines Resistance to Helicoerpa armigera in Upland Cotton Baolong Zhang, Wangzhen Guo, and Tianzhen Zhang Part IV. Application
15. Agrobacterium rhizogenes-Induced Cotton Hairy Root Culture as an Alternative Tool for Cotton Functional Genomics Hee Jin Kim
16. Overexpression of miR 156 in Cotton via Agrobacterium-Mediated Transformation Baohong Zhang, Min Wang, Xin Zhang, Chengqi Li, and Qinglian Wang
17. Development of Transgenic CryIA(c)+GNA Cotton Plants via Pollen Tube Pathway Method Confers Resistance to Helicoverpa armigera and Aphis gossypii Glover Zhi Liu, Zhen Zhu, and Tianzhen Zhang
18. Agrobacterium-Mediated Transformation of Cotton (Gossypium hirsutum) Shoot Apex with a Fungal Phytase Gene Improves Phosphorus Acquisition Zhiying Ma, Jianfeng Liu, and Xingfen Wang
19. Genetic Transformation of Cotton with a Harpin-Encoding Gene hpaXoo Confers an Enhanced Defense Response Against Verticillium dahliae Kleb Weiguo Miao and Jingsheng Wang
20. Development of Insect-Resistant Transgenic Cotton with Chimeric TVip3A* Accumulating in Chloroplasts Jiahe Wu and Yingchuan Tian Part V. Risk Assessment
21. Determining Gene Flow in Transgenic Cotton Xiaoping Pan

Customer Reviews