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Advanced Protocols in Oxidative Stress II

Handbook / Manual

Series: Methods in Molecular Biology Volume: 594

Edited By: Donald Armstrong

442 pages, 139 illus

Humana Press

Hardback | Dec 2009 | #182839 | ISBN-13: 9781607614104
Availability: Usually dispatched within 1 week Details
NHBS Price: £108.00 $143/€121 approx

About this book

Expanding upon the research elucidated by the first volume of this collection, this title presents thirty additional cutting-edge chapters focusing on novel techniques for detecting ROS/RNS, unique AOX technology and applications, gene expression and biostatistics for evaluating OS-derived experimental data. The international panel of authors also provide animal models and numerous studies concentrating on mitochondria during hypoxic conditions using advanced methods for pO2, peroxynitrate, reactive S-nitrosothiols, lipid peroxides, COX, and the mitochondrial membrane potential.


Part I: Reactive Oxygen and Nitrogen Techniques 1. Current Status of Measuring Oxidative Stress Beniamino Palmieri and Valeriana Sblendorio 2. PO2 and ROS/RNS Measurements in the Microcirculation in Hypoxia Silvia Bertuglia and Marcos Intaglietta 3. Nitrate Reductase Activity of Mitochondrial Aldehyde Dehydrogenase (ALDH-2) as a Redox Sensor for Cardiovascular Oxidative Stress Andreas Daiber and Thomas Munzel 4. Identification of ROS Using Oxidized DCFDA and Flow-Cytometry Evgeniy Eruslanov and Sergei Kusmartsev 5. Measurement of 8-Isoprostane in Exhaled Breath Condensate Paolo Montuschi, Peter J. Barnes, and Giovanni Ciabattoni 6. Electron Paramagnetic Resonance Oximetry and Redoximetry Guanglong He 7. Measurement of Mitochondrial Membrane Potential and Proton Leak Gaetano Serviddio and Juan Sastre 8. Determination of Erythrocyte Fragility as a Marker of Pesticide-Induced Membrane Oxidative Damage Bechan Sharma, Devendra K. Rai, Prashant Kumar Rai, S.I. Rizvi, and Geeta Watal 9. Using N, N, N', N'-Tetramethyl-p-phenylenediamine (TMPD) to Assay Cyclooxygenase Activity In Vitro Nenad Petrovic and Michael Murray 10. Structural and Functional Changes in the Insulin Molecule Produced by Oxidative Stress Rafael Medina-Navarro, Alberto M. Guzman-Grenfell, Ivonne Olivares-Corichi, and Juan J. Hicks 11. Multiphoton Redox Ratio Imaging for Metabolic Monitoring in vivo Melissa Skala and Nirmala Ramanujam 12. Using Fluorescence-Activated Flow Cytometry to Determine Reactive Oxygen Species Formation and Membrane Lipid Peroxidation in Viable Boar Spermatozoa H. David Guthrie and Glenn R. Welch 13. Lipofuscin: Detection and Quantification by Microscopic Techniques Tobias Jung, Annika Hohn, and Tilman Grune Part II: Antioxidant Technology and Application 14. Oxy-Score: A Global Index to Improve Evaluation of Oxidative Stress by Combining Pro- and Antioxidant Markers Fabrizio Veglia, Viviana Cavalca, and Elena Tremoli 15. CUPRAC (Cupric Ion Reducing Antioxidant Capacity) Assay for Antioxidants in Human Serum and for Hydroxyl Radical Scavengers Resat Apak, Kubilay Guclu, Mustafa Ozyurek, Burcu Bektasoglu, and Mustafa Bener 16. Analysis of Antioxidant Activities in Vegetable Oils and Fat Soluble Vitamins and Biofactors by the PAO-SO Method Kazuo Sakai, Satoko Kino, Masao Takeuchi, Tairin Ochi, Giuseppe Da Cruz, and Isao Tomita 17. Measuring Antioxidant Capacity Using the ORAC and TOSC Assays Andrew R. Garrett, Byron K. Murray, Richard A. Robison, and Kim L. O'Neill 18. Assessing the Neuroprotective Effect of Antioxidant Food Factors by Application of Lipid-Derived Dopamine Modification Adducts Xuebo Liu, Naruomi Yamada, and Toshihiko Osawa 19. LIBS-Based Detection of Antioxidant Elements: A New Strategy Geeta Watal, Bechan Sharma, Prashant Kumar Rai, Dolly Jaiswal, Devendra K. Rai, Nilesh K. Rai, and A.K. Rai 20. A Method for Evaluation of Antioxidant Activity Based on Inhibition of Free Radical-Induced Erythrocyte Hemolysis Jun Takebayashi, Jianbin Chen, and Akihiro Tai 21. Design and Synthesis of Antioxidant a-Lipoic Acid Hybrids Maria Koufaki and Anastasia Detsi 22. Characterization of the Antioxidant Properties of Pentaerithrityl Tetranitrate (PETN)-Induction of the Intrinsic Antioxidative System Heme Oxygenase-1 (HO-1) Andreas Daiber and Thomas Munzel 23. Direct Determination of Tissue Aminothiol, Disulfide, and Thioether Levels Using HPLC-ECD with a Novel Stable Boron-Doped Diamond Working Electrode Bruce Bailey, John Waraska, and Ian Acworth 24. Activation of Erythrocyte Plasma Membrane Redox System Provides a Useful Method to Evaluate Antioxidant Potential of Plant Polyphenols Syed Ibrahim Rizvi, Rashmi Jha, and Kanti Bhooshan Pandey 25. Antioxidant Activity of Biotransformed Sex Hormones Facilitated by Bacillus Stearothermophilus Mohammad Afzal, Sameera Al-Awadi, and Sosamma Oommen 26. Separation of Phenylpropanoids and Evaluation of Their Antioxidant Activity Sammer Yousuf, M. Iqbal Choudhary, and Atta-Ur-Rahman Part III: Gene Expression 27. Generation of Antioxidant Adenovirus Gene Transfer Vectors Encoding CuZnSOD, MnSOD, and Catalase Aoife M. Duffy, Timothy O'Brien, and Jillian M. McMahon 28. HPLC Purification of Adenoviral Vectors Marc Eglon, Barry McGrath, and Timothy O'Brien 29. Mapping of Oxidative Stress Response Elements of the Caveolin-1 Promoter Ferruccio Galbiati and Janine N. Bartholomew Part IV: Biostatistics 30. Meta-Analysis: Drawing Conclusions When Study Results Vary Leslie Rosenthal and Enrique Schisterman

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