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About this book
About this book
It has been recently realized that cells often indulge in self-destruction ("suicide") and can be encouraged to do so which is useful for treatment of cancer. Regulated cell death is also vital for the correct development of organisms. This exciting area of research is described by internationally recognized authorities who provide the background and precise procedures how to perform these studies.
Preface; 1. Overview of Apoptosis; 2. The Morphological Recognition of Apoptosis; 3. Assessment of DNA Damage in Apoptosis; 4. Analysis of Cell Death by Flow and Laser Scanning Cytometry; 5. Cell-mediated Cytotoxicity and Cell Death Receptors; 6. Sphingolipids as Messengers of Cell Death; 7. Cytochemical Detection of Cyto- and Nucleoskeletal Changes During Apoptosis; 9. Methods of Measuring Bcl-2 Family Proteins and Their Functions; 10. Methods for Detecting Proteolysis During Apoptosis in Intact Cells; Index
"Ten chapters by contributors from leading laboratories globally provide a comprehensive guide to the latest techniques for studying cell death by apoptosis. Early chapters distinguish apoptosis for normal cell population control from necrosis and non-specific cell DNA damage. Later chapters cover step-by-step use of key investigation methods, particularly signaling, the apoptotic cascade, and the execution of apoptosis. Topics include: assessment of DNA damage, analysis by flow and laser-scanning cytometry, sphingolipids as messengers of cell death, and associated metabolic alterations. Includes microscopy images, and a list of US and UK lab suppliers. The editor is affiliated with the New Jersey Medical School."--SciTech Book News