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Applied Microbial Physiology: A Practical Approach

Handbook / Manual

Series: Practical Approach Series Volume: 183

Edited By: PM Rhodes and PF Stanbury

270 pages, Illus, tabs

IRL Press

Paperback | Jul 1997 | #82029 | ISBN: 0199635773
Availability: Usually dispatched within 2-3 weeks Details
NHBS Price: £69.99 $92/€80 approx
Hardback | Dec 1997 | #83941 | ISBN: 0199635781
Out of Print Details

About this book

The rapid growth in biotechnology in recent years has led to an upsurge in interest in microbial technology amongst many biochemists, molecular biologists, geneticists, virologists, endocrinologists, and clinicians. Their objectives may be very diverse, ranging from the isolation of a stable enzyme from a hyperthermophile to the expression of a human protein by a recombinant yeasts or bacterium. Advance in microbial physiology have made possible a rational approach to optimization of product yield based on analysis of cultures, growth kinetics, and biochemical pathways. The application of statistical optimization methods, widely used in other fields, also has much to offer microbiology and biotechnology. The choice of material for this book has been influenced by both the need for practical information to enable to the isolation, handling, and culture of organisms and the necessity to generate and analyse data enabling the development of a process. It therefore contains chapters covering the 'husbandry' of microbiology, the generation of data by chemical and physical analysis, and the interpretation of such data. Data interpretation is considered from two points of view. Kinetic analyses of growth and product formation have frequently illuminated the development of fermentation processes. More recently, the analysis of the flux of metabolites through intermediate biochemical pathways has shown up important factors in metabolic engineering through the application of molecular biology techniques in microbial physiology. Applied Microbial Culture: A Practical Approach is a useful resource and guide to the successful culture of microorganisms in pure form, optimizing the culture conditions, and the scaling-up process to enable more detailed study.

"The editors note that during the rapid development of biotechnology, scientists from many biological or medical disciplines have come to rely on the use of microorganisms to produce substances of commercial value. In many cases, the scientists do not have the training of a microbiologist, and this book seeks to provide the type of information that will enable them to move from research results to the point of production. . . . The contributors are well-known scientists in their fields. There are two appendixes, a useful list of 66 suppliers of equipment and chemicals . . . and an extensive list of the larger culture collections around the world. . . . All of the chapters are well written, and the book gives a good general perspective of the immense complexity of biotechnology."--The Quarterly Review of Biology


1. Sources of microorganisms and their preservation; 2. Isolation and growth of hyperthermophiles; 3. Design and optimization of growth media; 4. Working with laboratory fermenters; 5. Measurement of biomass; 6. Fermenter vent gas analysis; 7. The analytical chemistry of microbial cultures; 8. Analysis of microbial growth data; 9. Metabolic flux analysis of fermentation processes; Appendix 1. Addresses of culture collections, microbial databases and national and international culture collection organizations; Appendix 2. Suppliers of specialist items

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