About this book
Provides a carefully selected collection of techniques for the microbiologist wishing to study the interactions between bacteria and their targets, whether human, animal, or plant.
Preface. S. Falkow and R.H. Valdivia, The Detection of Virulence Genes: P.V. Tearle, Laboratory Safety--Working With Pathogens: P. Vandamme, Detection, Identification, and Speciation: P. Vandamme, Introduction. M. Ieven, Detection. P. Vandamme, Speciation. P. Vandamme, Identification. T. Mitchell, Host Interactions--Animals: T.J. Mitchell, Introduction. A.D. Jackson, R.B. Dowling, and R. Wilson, Interaction of Bacteria and Their Products with Tissues in Organ Culture. B. Burke, Transgenic and Knockout Animals in the Study of Bacterial Pathogenesis. T.S. Wallis, Interactions of Bacteria and their Products in Whole Animals Systems. D. Charboneau and J.B. Rubins, Interaction of Bacteria and their Products with Tissue Culture Cells. M. Daniels, Host Interactions--Plants: M.J. Daniels, Introduction. M.J. Daniels, Testing Pathogenicity. A. Collmer and S.V. Beer, hrp Genes and Their Function. U. Bonas, Avirulence Genes. B. Py, F. Barras, S. Harris, N. Robson, and G.P.C. Salmond, Extracellular Enzymes and Their Role in Erwinia Virulence. C.L. Bender, Bacterial Phytotoxins. S.E. Lindow, Epiphetic Growth and Survival. P. Williams, Biochemical Approaches: K. Hardie and P. Williams, Introduction: Fractionation of Bacterial Cell Envelopes. C.D. O'Connor, M. Farris, L.G. Hunt, and J.N. Wright, The Proteome Approach. N.B.L. Powell, Microscopic Approaches to the Study of Bacterial Cell Associated Virulence Determinants. J. Holland and A. Gorringe, Characterization of Bacterial Surface Receptor-Ligand Interactions. R.E. Sockett, Characteristic Flagella and Motile Behaviour. P. Klemm, M. Schembri, B. Stentebjerg-Olesen, H. Hasman, and D.L. Hasty, Fimbriae: Detection, Purification, and Characterization. C. Whitfield and M.B. Perry, Isolation and Purification of Cell Surface Polysaccharides from Gram-negative Bacteria. C. Whitfield and M.B. Perry, Structural Analysis of Cell Surface Polysaccharides from Gram-negative Bacteria. K.D. Young, Techniques for Analysis of Peptidoglycans. M. Moayeri and R.A. Welch, Bacterial Ecotoxins. A. Filloux and K.R. Hardie, A Systematic Approach to the Study of Protein Secretion in Gram-negative Bacteria. M. Camara, M. Daykin, and S.R. Chhabra, Detection, Purification, and Synthesis of N-acylhomoserine Lactone Quorum Sensing Signal Molecules. A.W. Smith, Iron Starvation and Siderophore-Mediated Iron Uptake. C. Dorman, Molecular Genetic Approaches: C. Dorman, Introduction. J.C.D. Hinton, Genetic Approaches to the Study of Pathogenic Salmonellae. D.W. Holden and M. Hensel, Signature Tagged Mutagenesis. S.-L. Lui and K.E. Sanderson, Bacterial Genome Analysis. H. Yu, J.C. Boucher, and V. Deretic, Molecular Analysis of Pseudomonas Aeruginosa Virulence. V. Scarlato, D. Beier, and R. Rappuoli, Molecular Genetics of Bordetella Pertussis Virulence. A.H.M. van Vliet, A.C. Wood, J. Henderson, K. Woolridge, and J.M. Ketley, Genetic Manipulation of Enteric Campylobacter Species. S. Schaferkordt, E. Domann, and T. Chakraborty, Molecular Approaches for the Study of Lysteria. T.J. Foster, Molecular Genetic Analysis of Staphylococcal Virulence. M.A. Scidmore, J. Bannantine, and T. Hackstadt, Molecular Approaches to Studying Chlamydia. I. Roberts, Gene Expression and Analysis: M. Roberts, Host Reactions--Animals and Humans: I. Rosenshine, Cytoskeletal Rearrangements Induced by Bacterial Pathogens. F.D. Menozzi and C. Locht, Strategies for the Study of the Interactions Between Bacterial Adhesins and Glycosaminoglycans. P. Everest, M. Roberts, and G. Dougan, Strategies for the Use of Gene Knockout Mice in the Investigation of Bacterial Pathogenesis and Immunology. J.W. Mansfield, C.S. Bestwick, I.R. Brown, B. Boher, M. Nicole, and M. Essenberg, Host Reactions--Plants: A. Cravioto, Strategies and Problems for Disease Control: A. Cravioto, Introduction. A. Cravioto, R. Cabrera, C. Eslava, and Y. Lopez-Vidal, Strategies for Control of Common Infectious Diseases Prevalent in Developing Countries. C.F. Amabile-Cuevas, R. Cabrera, L.Y. Fuchs, and F. Valenzuela, Antibiotic Resistance and Prescription Practices in Developing Countries. M. Pallen, Internet Resources for Research on Bacterial Pathogenesis. Subject Index.