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With the dwindling supplies of fossil fuels and growing concerns regarding climate changes due to greenhouse gases from these fuels, public opinion has swung dramatically towards favoring the development of renewable energy sources. In "Biofuels: Methods and Protocols", career-long experts explore a full range of methods for bioenergy covering important topics such as biomass production and delivery to the biorefinery, detailed biochemical characterization, as well as biotechnological techniques for converting plant matter into fuels and chemicals. Time is of the essence in this field, and this volume aims to provide direction and assistance to the growing cadre of researchers endeavoring to develop new sources of bioenergy with a solid, easy-to-use collection of tried-and-true methods which will save time and effort in the field and the laboratory.
Written in the highly successful "Methods in Molecular Biology" series format, chapters include brief introductions to their respective topics, lists of the necessary equipment, materials and reagents, step-by-step, readily reproducible field and laboratory protocols, and notes on troubleshooting and avoiding common pitfalls. Timely and authoritative, "Biofuels: Methods and Protocols" seeks to help scientists and engineers as they develop and optimize bioenergy technologies needed to drastically change the course of our energy future as soon as possible.
1. Biomass Supply Logistics and Infrastructure Shahabaddine Sokhansanj and J. Richard Hess 2. Selecting, Establishing, and Managing Switchgrass (Panicum virgatum) for Biofuels David J. Parrish and John H. Fike 3. Agronomic Experiences with Miscanthus x giganteus in Illinois, USA Richard Pyter, Emily Heaton, Frank Dohleman, Tom Voigt, and Stephen Long 4. Genetic Transformation of Switchgrass Yajun Xi, Yaxin Ge, and Zeng-Yu Wang 5. Lignocellulosic Biomass Pretreatment Using AFEX Venkatesh Balan, Bryan Bals, Shishir P. S. Chundawat, Derek Marshall and Bruce E. Dale 6. Pretreatment of Biomass by Aqueous Ammonia for Bioethanol Production Tae Hyun Kim, Rajesh Gupta, and Y. Y. Lee 7. Liquid Hot Water Pretreatment of Cellulosic Biomass Youngmi Kim, Rick Hendrickson, Nathan S. Mosier, and Michael R. Ladisch 8. Dilute Acid and Autohydrolysis Pretreatment Bin Yang and Charles E. Wyman 9. Lime Pretreatment Rocio Sierra, Cesar Benigno Granda, and Mark Thomas Holtzapple 10. Analytical Characterization of Fermentation Inhibitors in Biomass Pretreatment Samples Using Liquid Chromatography, UV-Visible Spectroscopy, and Tandem Mass Spectrometry Lekh N. Sharma, Christopher Becker, and C. Kevin Chambliss 11. Biomass Compositional Analysis for Energy Applications Bonnie R. Hames 12. High Throughput Screening of Plant Cell Wall Composition Using Pyrolysis Molecular Beam Mass Spectroscopy Robert Sykes, Matthew Yung, Evandro Novaes, Matias Kirst, Gary Peter, and Mark Davis 13. Preparation and Analysis of Biomass Lignins Alicia L. Compere and William L. Griffith 14. Cellulase Assays Y.-H. Percival Zhang, Jiong Hong, and Xinhao Ye 15. Assessing Cellulase Performance On Pretreated Lignocellulosic Biomass Using Saccharification and Fermentation-Based Protocols Nancy Dowe 16. Basic Laboratory Culture Methods for Anaerobic Bacteria Herbert J. Strobel 17. Simultaneous Saccharification and Fermentation and Partial Saccharification and Co-Fermentation of Lignocellulosic Biomass for Ethanol Production Joy Doran-Peterson, Amruta Jangid, Sarah K. Brandon, Emily DeCrescenzo-Henriksen, Bruce Dien, and Lonnie O. Ingram 18. Biodiesel: Small Scale Production and Quality Requirements Jon Van Gerpen
From the reviews: "Bioenergy research, development and industrialization are moving at a rapid pace. ! This book is therefore timely and focuses on lignocellulose-to-bioethanol technologies, with particular emphasis on maize residues and 'energy crops' ! . Biomass supply, pretreatment and analysis are well covered ! . This is a most useful text, or ! lab handbook, that covers many analytical methods and applications for biomass-to-biofuel technologies." (Graeme Walker, Microbiology Today, June, 2010)