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The book comprises ten chapters written by experts in the field on cell-cell interactions and their role in biology and medicine. Cell-cell interactions are the means by which cells are able to communicate, transfer information, develop spatial awareness and coordinate their differentiation. The ten areas have been selected for their breadth and relevance to modern research scientists where cell-cell interactions have been shown to play a critical role in biological processes. Chapters include methodologies for specialised cell types (keratinocytes, leukocytes, neurons, endothelial cells), specialised domains of membrane contact (adhesion molecules, gap junctions, tight junctions), different developmental models (Drosophila, Xenopus, mouse) and, for mammalian development, distinct stages of medical importance (oocyte-granulosa cells; preimplantation embryos; implantation). Throughout, molecular, cellular, biochemical and physiological protocols are provided in comprehensive detail to help the reader develop new skills and understanding.
1. Cadherin adhesion regulation in keratinocytes; 2. Analysis of intercellular adhesion molecules in endothelial cells; 3. Gap junction mediated interactions between cells; 4. Functional analysis of the tight junction; 5. Leukocyte cell adhesion interactions; 6. Studying cell interactions during development of the nervous system in Drosophila; 7. Tight junction protein expression in early Xenopus development and protein interaction studies; 8. Oocyte-granulosa cell interactions; 9. Cell-cell interactions in early mammalian development; 10. Model Systems for the investigation of implantation
... this volume fits well on the shelves reserved for the Practical Approach Series in each professional scientific library Nature cell Biology