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Academic & Professional Books  Organismal to Molecular Biology  Biochemistry & Molecular Biology

Cell Separation: A Practical Approach

Handbook / Manual
Series: Practical Approach Series Volume: 193
Edited By: Derek Fisher, Gillian E Francis and David Rickwood
288 pages, Illus
Publisher: IRL Press
Cell Separation: A Practical Approach
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  • Cell Separation: A Practical Approach ISBN: 9780199635795 Paperback Nov 1998 Usually dispatched within 2-3 weeks
    £69.99
    #83406
  • Cell Separation: A Practical Approach ISBN: 9780199635801 Hardback Dec 1998 Out of Print #83404
Selected version: £69.99
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About this book

Isolating specific cells or fractionating viable cell populations is an essential step of many of the techniques used across a wide range of biological and related disciplines. This text aims to inform those working in all areas of the biological sciences of the methods which are available to them and which is likely to be the most useful. The contributing authors have provided a comprehensive guide to the methods used to prepare cell suspensions and to separate viable cells depending on their different characteristics - such as size, density, surface charge and immunological identity. Each chapter offers direct practical guidance for the various techniques and describes the advantages and limitations of each. Reproducible protocols, hints and tips for success and troubleshooting suggestions are provided.

Contents

1. Preparation of single cell suspensions; 2. Fractionation of cells by sedimentation methods; 3. Centrifugal elutriation; Appendix: Simulation of centrifugal elutriation for the Beckman E-6B chamber; 4. Separation and frustrationation of cells by partitioning in aqueous two-phase systems; Appendix: Manually operated counter current distribution apparatus; 5. Separation cells by flow cytometry; 6. Immuno methods: magnetic, columns and panning techniques; 7. Separation of cells using free flow electrophoresis; Appendix: Suppliers

Customer Reviews

Handbook / Manual
Series: Practical Approach Series Volume: 193
Edited By: Derek Fisher, Gillian E Francis and David Rickwood
288 pages, Illus
Publisher: IRL Press
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