Chemistry of Protein Conjugation and Crosslinking

About this book

Chemical cross-linking reagents have attained great practical use in industry as well as in basic research, and an understanding of their fundamental principles of reaction is paramount to their applications. With broad coverage of the development and application of these reagents, "Chemistry of Protein Conjugation and Cross-Linking" discusses the mechanism of reaction and allows you to put the theory into practice. The book offers an explanation of the underlying mechanism of chemical modification, surveys all the bifunctional reagents used in bioconjugation and cross-linking, and provides a review of practical applications of these reagents in various areas of biochemistry, molecular biology, biotechnology, nucleic acid chemistry, immunochemistry, and diagnostic and biomedical disciplines. It contains numerous examples and illustrations, plus step-by-step explanations to reaction procedures. It is an excellent introduction and a comprehensive reference about chemical modification.

Contents

Introduction. Reactive Groups of Proteins and Their Modifying Agents. Choice and Design of Cross-Linking Reagents. Homobifunctional Cross-Linking Reagents. Heterobifunctional Cross-Linkers. Zero-Length Cross-Linking Reagents. Procedures, Analysis, and Complications. Application of Chemical Cross-Linking to Soluble Proteins. Cross-Linking of Membrane Proteins. Preparation of Enzyme Immunoconjugates and Other Immunoassay Components. Preparation of Immunotoxins and Other Drug Conjugates for Targeting Therapeutics. Conjugation of Proteins to Solid Matrices. Other Applications and Future Prospects.

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